Yu Zhixue, Wang Hui, He Yue, Chen Dongfei, Chen Ruipeng, Tang Xiangfang, Zhou Mengting, Yao Junhu, Xiong Benhai
State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
College of Animal Science and Technology, Northwest A&F University, Xianyang 712100, China.
Int J Mol Sci. 2024 Dec 8;25(23):13204. doi: 10.3390/ijms252313204.
Monitoring potassium ion (K) concentration is essential in veterinary medicine, particularly for preventing hypokalemia in dairy cows, which can severely impact their health and productivity. While traditional laboratory methods like atomic absorption spectrometry are accurate, they are also time-consuming and require complex sample preparation. Ion-selective electrodes (ISEs) provide an alternative that is faster and more suitable for field measurements, but their performance is often compromised under variable temperature conditions, leading to inaccuracies. To address this, we developed a novel screen-printed ion-selective electrode (SPE) with hydrophobic TiC Mxene and gold nanoparticles (AuNPs), integrated with a temperature sensor. This design improves stability and accuracy across fluctuating temperatures by preventing water layer formation and enhancing conductivity. The sensor was validated across temperatures from 5 °C to 45 °C, achieving a linear detection range of 10⁵ to 10 M and a response time of approximately 15 s. It also demonstrated excellent repeatability, selectivity, and stability, making it a robust tool for K monitoring in complex environments. This advancement could lead to broader applications in other temperature-sensitive analytical fields.
监测钾离子（K）浓度在兽医学中至关重要，特别是对于预防奶牛低钾血症，因为低钾血症会严重影响奶牛的健康和生产力。虽然像原子吸收光谱法这样的传统实验室方法很准确，但它们也很耗时，并且需要复杂的样品制备。离子选择性电极（ISE）提供了一种更快且更适合现场测量的替代方法，但其性能在可变温度条件下往往会受到影响，导致测量不准确。为了解决这个问题，我们开发了一种新型的丝网印刷离子选择性电极（SPE），它采用了疏水的TiC MXene和金纳米颗粒（AuNP），并集成了一个温度传感器。这种设计通过防止水层形成和提高导电性，在波动温度下提高了稳定性和准确性。该传感器在5°C至45°C的温度范围内进行了验证，实现了10⁵至10 M的线性检测范围和约15秒的响应时间。它还表现出出色的重复性、选择性和稳定性，使其成为复杂环境中钾监测的强大工具。这一进展可能会在其他对温度敏感的分析领域得到更广泛的应用。
