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开发和评估一种针对 B 族感染的多表位亚单位疫苗。

Development and evaluation of a multi-epitope subunit vaccine against group B infection.

机构信息

College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, People's Republic of China.

OIE Reference Lab for Swine Streptococcosis, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, People's Republic of China.

出版信息

Emerg Microbes Infect. 2022 Dec;11(1):2371-2382. doi: 10.1080/22221751.2022.2122585.

DOI:10.1080/22221751.2022.2122585
PMID:36069613
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9543083/
Abstract

(Group B , GBS) is a multi-host pathogen, even causing life-threatening infections in newborns. Vaccination with GBS crossed serotypes vaccine is one of the best options for long-term infection control. Here we built a comprehensive epitope-prediction workflow pipeline to design a multivalent ultiepitope-based subunit accine containing 11 epitopes against (MVSA). All epitopes in MVSA came from the proteins which were antigenic-confirmed, virulent-associated, surface-exposed and conserved in ten GBS serotypes. The analysis showed MVSA had potential to evoke strong immune responses and enable worldwide population coverage. To validate MVSA protection efficacy against GBS infection, immune protection experiments were performed in a mouse model. Importantly, MVSA induced a high titre of antibodies, significant proliferation of mice splenocytes and elicited strong protection against lethal-dose challenge with a survival rate of 100% in mice after three vaccinations. Meanwhile, the polyclonal antibody against MVSA did not only inhibit for growth of GBS from six crucial serotypes but also protect 100% naive mice from GBS lethal challenge. These active and passive immunity assay results suggested that MVSA could therefore be an efficacious multi-epitope vaccine against GBS infection.

摘要

（B 群链球菌，GBS）是一种多宿主病原体，甚至会导致新生儿发生危及生命的感染。接种 GBS 交叉血清型疫苗是长期感染控制的最佳选择之一。在这里，我们建立了一个全面的表位预测工作流程管道，以设计一种包含 11 个针对（MVSA）表位的多价超表位基于亚单位的疫苗。MVSA 中的所有表位均来自抗原性确认、毒力相关、表面暴露和 10 种 GBS 血清型中保守的蛋白。分析表明，MVSA 有可能引发强烈的免疫反应，并能覆盖全球人口。为了验证 MVSA 对 GBS 感染的保护效力，我们在小鼠模型中进行了免疫保护实验。重要的是，MVSA 诱导了高滴度的抗体，显著促进了小鼠脾细胞的增殖，并在三次接种后对致死剂量的 GBS 攻击产生了强烈的保护作用，小鼠的存活率为 100%。同时，针对 MVSA 的多克隆抗体不仅抑制了来自六个关键血清型的 GBS 的生长，而且还保护了 100%的未感染 GBS 的小鼠免受致命性挑战。这些主动和被动免疫检测结果表明，MVSA 因此可能成为一种有效的针对 GBS 感染的多表位疫苗。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d04/9543083/eafdc36412d4/TEMI_A_2122585_F0006_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d04/9543083/cf153ad2f953/TEMI_A_2122585_F0001_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d04/9543083/f4e731fe59d7/TEMI_A_2122585_F0002_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d04/9543083/d04c1117cf43/TEMI_A_2122585_F0003_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d04/9543083/bf0087f62fdb/TEMI_A_2122585_F0004_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d04/9543083/0bf640733568/TEMI_A_2122585_F0005_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d04/9543083/eafdc36412d4/TEMI_A_2122585_F0006_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d04/9543083/cf153ad2f953/TEMI_A_2122585_F0001_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d04/9543083/f4e731fe59d7/TEMI_A_2122585_F0002_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d04/9543083/d04c1117cf43/TEMI_A_2122585_F0003_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d04/9543083/bf0087f62fdb/TEMI_A_2122585_F0004_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d04/9543083/0bf640733568/TEMI_A_2122585_F0005_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d04/9543083/eafdc36412d4/TEMI_A_2122585_F0006_OC.jpg

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