Zhang Rui, Dai Lu, Jia Yizhen, Qi Haoran, He Junfeng, Cheng Jiaxin, Gao Xuejun, Lei Liancheng, Liu Feng
College of Animal Science and Technology, Yangtze University, Jingzhou, Hubei, China.
College of Veterinary Medicine, Jilin University, Changchun, China.
Front Immunol. 2025 Sep 1;16:1652907. doi: 10.3389/fimmu.2025.1652907. eCollection 2025.
() is the pathogen responsible for swine pasteurellosis, which can impede their growth and even cause death, leading huge economic losses to the global pig industry. can be divided into 5 serotypes, and existing vaccines have low cross-immunity protection. Therefore, developing a vaccine that can provide effective cross-protection is essential for preventing swine pasteurellosis and reducing the abuse of antibiotics. In this study, six dominant antigenic proteins of (PlpE, OmpA, OmpH, VacJ, Omp87 and Cp39) were selected. Through bioinformatics methods, 20 B-cell epitopes, 7 CTL epitopes and 11 Th-cell epitopes were predicted. The multi-epitope antigen PME was constructed by connecting these epitopes with linkers, and then the recombinant protein His-PME and the recombinant plasmid pcDNA3.1-PME could effectively stimulate immunized mice to produce antibodies, IL-4 and IFN-γ. The protection rates of His-PME group and pcDNA3.1-PME group were 62.5% and 75% against serotype A, and 87.5% and 100% against serotype D, respectively. Furthermore, the pathological lung damages in the His-PME group and the pcDNA3.1-PME group were significantly alleviated, and the bacterial loads in lung tissues were significantly decreased. These results indicated that the subunit vaccine His-PME and the DNA vaccine pcDNA3.1-PME can effectively resist the infection of and have good immunogenicity and cross-protection. Therefore, the multi-epitope vaccine PME can be regarded as a candidate vaccine for the prevention of infection.
（某病原体）是猪巴氏杆菌病的致病原，可阻碍猪的生长甚至导致死亡，给全球养猪业造成巨大经济损失。（该病原体）可分为5个血清型，现有疫苗的交叉免疫保护作用较低。因此，开发一种能提供有效交叉保护的疫苗对于预防猪巴氏杆菌病和减少抗生素滥用至关重要。在本研究中，选取了（某病原体）的6种主要抗原蛋白（PlpE、OmpA、OmpH、VacJ、Omp87和Cp39）。通过生物信息学方法，预测了20个B细胞表位、7个CTL表位和11个Th细胞表位。通过连接子连接这些表位构建了多表位抗原PME，然后重组蛋白His-PME和重组质粒pcDNA3.1-PME能有效刺激免疫小鼠产生抗体、IL-4和IFN-γ。His-PME组和pcDNA3.1-PME组对A型（某病原体）的保护率分别为62.5%和75%，对D型（某病原体）的保护率分别为87.5%和100%。此外，His-PME组和pcDNA3.1-PME组的肺部病理损伤明显减轻，肺组织中的细菌载量显著降低。这些结果表明，亚单位疫苗His-PME和DNA疫苗pcDNA3.1-PME能有效抵抗（某病原体）的感染，具有良好的免疫原性和交叉保护作用。因此，多表位疫苗PME可被视为预防（某病原体）感染的候选疫苗。
