Institute of Physical Chemistry of the Polish Academy of Sciences, ul. Kasprzaka 44/52, 01-224 Warszawa, Poland.
Broad Institute of MIT and Harvard, Merkin Building, 415 Main St, Cambridge, MA 02142, USA.
Lab Chip. 2022 Sep 27;22(19):3637-3662. doi: 10.1039/d2lc00394e.
The rise of antibiotic resistance is a threat to global health. Rapid and comprehensive analysis of infectious strains is critical to reducing the global use of antibiotics, as informed antibiotic use could slow down the emergence of resistant strains worldwide. Multiple platforms for antibiotic susceptibility testing (AST) have been developed with the use of microfluidic solutions. Here we describe microfluidic systems that have been proposed to aid AST. We identify the key contributions in overcoming outstanding challenges associated with the required degree of multiplexing, reduction of detection time, scalability, ease of use, and capacity for commercialization. We introduce the reader to microfluidics in general, and we analyze the challenges and opportunities related to the field of microfluidic AST.
抗生素耐药性的上升对全球健康构成威胁。快速全面地分析传染病菌株对于减少全球抗生素的使用至关重要，因为合理使用抗生素可以减缓全球耐药菌株的出现。已经开发出多种使用微流体解决方案的抗生素药敏测试 (AST) 平台。在这里，我们描述了有助于 AST 的微流体系统。我们确定了在克服与所需的多重检测程度、缩短检测时间、可扩展性、易用性和商业化能力相关的突出挑战方面的关键贡献。我们向读者介绍了微流控技术，并分析了与微流控 AST 领域相关的挑战和机遇。
