Chen Jiahong, Zhong Jianwei, Chang Yifu, Zhou Yinning, Koo Seok Hwee, Tan Thean Yen, Lei Hongtao, Ai Ye
Guangdong Provincial Key Laboratory of Food Quality and Safety/National-Local Joint Engineering Research Center for Machining and Safety of Livestock and Poultry Products, College of Food Science, South China Agricultural University, Guangzhou, 510642, China.
Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, 510642, China.
Small. 2024 Feb;20(6):e2303352. doi: 10.1002/smll.202303352. Epub 2023 Oct 4.
Antimicrobial resistance has become a serious threat to the global public health. Accurate and rapid antimicrobial susceptibility testing (AST) allows evidence-based prescribing of antibiotics to improve patient care and clinical outcomes. Current culture-based AST assays are inherently limited by the doubling time of bacterial reproduction, which require at least 24 h to have a decisive result. Herein, a label-free electrical impedance-based microfluidic platform designed to expedite and streamline AST procedure for clinical practice is presented. Following a 30-min exposure of bacterial samples to antibiotics, the presented high-throughput, single-bacterium level impedance characterization platform enables a rapid 2-min AST assay. The platform facilitates accurate analysis of individual bacterial viability, as indicated by changes in electrical characteristics, thereby enabling the determination of antimicrobial resistance. Moreover, the potential clinical applicability of this platform is demonstrated by testing different E. coli strains against five antibiotics, yielding 100% categorical agreements compared to standard culture methods.
抗菌药物耐药性已成为全球公共卫生的严重威胁。准确、快速的抗菌药物敏感性试验（AST）有助于基于证据开具抗生素处方，以改善患者护理和临床结果。当前基于培养的AST检测方法固有地受到细菌繁殖倍增时间的限制，至少需要24小时才能得出决定性结果。在此，我们展示了一种基于无标记电阻抗的微流控平台，旨在加快并简化用于临床实践的AST程序。在将细菌样本暴露于抗生素30分钟后，该高通量、单细菌水平的电阻抗表征平台能够在2分钟内快速完成AST检测。该平台有助于通过电特性变化准确分析单个细菌的活力，从而确定抗菌药物耐药性。此外，通过针对五种抗生素测试不同的大肠杆菌菌株，证明了该平台的潜在临床适用性，与标准培养方法相比，分类一致性达100%。
