血管化复合组织同种异体移植急性排斥反应中的 TRFs 和 tiRNAs 序列。
TRFs and tiRNAs sequence in acute rejection for vascularized composite allotransplantation.
机构信息
Department of Plastic Surgery, The Third Xiangya Hospital, Central South University, Changsha, Hunan, China.
Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
出版信息
Sci Data. 2022 Sep 7;9(1):544. doi: 10.1038/s41597-022-01577-y.
Illumina tRFs & tiRNAs-seq analysis was used to characterize the whole transcriptomes of acute rejection caused by vascularized composite allotransplantation (VCA). tRFs & tiRNAs-seq information for muscle samples with VCA was obtained and compared with similar information for same age- and sex-matched healthy control subjects. The expression of 16 tRFs and tiRNAs, including 5 up-regulated target genes and 11 down-regulated target genes, were significantly different. According to bioinformatics analysis and reverse transcription quantitative polymerase chain reaction, we speculate that tiRNA-1-34-Glu-CTC-1 plays an important role in VCA-induced acute rejection by regulating the CACNA1D gene in the MAPK signaling pathway The findings provide the whole-transcriptome signatures of acute rejection for VCA, allowing further exploration of gene expression patterns/signatures associated with the various clinical symptoms of acute rejection for VCA.
Illumina tRFs 和 tiRNAs-seq 分析用于描述血管化复合组织移植 (VCA) 引起的急性排斥反应的全转录组。获取了 VCA 肌肉样本的 tRFs 和 tiRNAs-seq 信息，并与同年龄和性别匹配的健康对照者的类似信息进行了比较。有 16 种 tRFs 和 tiRNAs 的表达存在显著差异，包括 5 个上调的靶基因和 11 个下调的靶基因。根据生物信息学分析和逆转录定量聚合酶链反应，我们推测 tiRNA-1-34-Glu-CTC-1 通过调节 MAPK 信号通路中的 CACNA1D 基因在 VCA 诱导的急性排斥反应中发挥重要作用。研究结果为 VCA 提供了急性排斥的全转录组特征，进一步探索了与 VCA 各种急性排斥临床症状相关的基因表达模式/特征。
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