Asante Ampadu Gertrude Adomaa, Mensah Jehoshaphat Oppong, Darko Godfred, Borquaye Lawrence Sheringham
Department of Chemistry, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
Central Laboratory, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
Evid Based Complement Alternat Med. 2022 Aug 28;2022:7211015. doi: 10.1155/2022/7211015. eCollection 2022.
This work focused on characterizing the chemical constituents and evaluating the antioxidant and antimicrobial activities of the essential oils obtained from the fruit and leaves of a flowering plant of the Anacardiaceae . Essential oils were extracted through steam distillation and characterized by gas chromatography-mass spectrometry. For the fruit essential oil, 35 compounds were obtained, and 25 compounds were identified in the leaf essential oil. The dominant compounds present in the fruit essential oil were ()-ethyl cinnamate (14.06%) and benzyl benzoate (12.27%). Methyl salicylate (13.05%) and heptacosane (12.69%) were the abundant compounds in the leaf essential oil. The antioxidant activity of the essential oils was evaluated via phosphomolybdenum, hydrogen peroxide scavenging, 2, 2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging, and thiobarbituric acid reactive substances (TBARS) assays. The total antioxidant capacity of fruit and leaf essential oils was 48.5 ± 0.7 g/gAAE and 48.5 ± 0.7 g/g AAE, respectively. The half maximal scavenging concentrations of the essential oils in the hydrogen peroxide; DPPH and TBARS assays ranged from 252.2 g/mL to 2288 g/mL. The antimicrobial activity of the essential oils was tested using broth dilution and disc diffusion assays against eight microorganisms. The essential oils exhibited broad-spectrum antimicrobial activity against the microorganisms with minimum inhibitory concentrations of 9.75-50 mg/mL. Also, the zones of inhibition of the oils ranged from 12 mm to 25 mm. The biofilm inhibitory activities of the oils were dose-dependent with BIC values of 42.49 ± 0.1 mg/mL and 97.34 ± 0.6 mg/mL for fruit and leaf essential oils, respectively. Molecular docking studies revealed that the antibiofilm action of the fruit and leaf essential oils could be due to inhibition of the quorum sensing protein, LasR. The results suggest a possible application of the oils as antioxidant and antimicrobial agents.
本研究聚焦于对漆树科一种开花植物的果实和叶片中提取的挥发油的化学成分进行表征，并评估其抗氧化和抗菌活性。通过水蒸气蒸馏法提取挥发油，并用气相色谱 - 质谱联用仪进行表征。从果实挥发油中获得了35种化合物，从叶片挥发油中鉴定出25种化合物。果实挥发油中的主要化合物为（）- 肉桂酸乙酯（14.06%）和苯甲酸苄酯（12.27%）。水杨酸甲酯（13.05%）和二十七烷（12.69%）是叶片挥发油中的主要成分。通过磷钼酸法、过氧化氢清除法、2,2 - 二苯基 - 1 - 苦基肼（DPPH）自由基清除法和硫代巴比妥酸反应物（TBARS）测定法评估挥发油的抗氧化活性。果实和叶片挥发油的总抗氧化能力分别为48.5±0.7 g/g AAE和48.5±0.7 g/g AAE。挥发油在过氧化氢、DPPH和TBARS测定中的半数最大清除浓度范围为252.2 g/mL至2288 g/mL。使用肉汤稀释法和纸片扩散法对8种微生物测试挥发油的抗菌活性。挥发油对这些微生物表现出广谱抗菌活性，最低抑菌浓度为9.75 - 50 mg/mL。此外，挥发油的抑菌圈范围为12 mm至25 mm。挥发油的生物膜抑制活性呈剂量依赖性，果实和叶片挥发油的生物膜抑制浓度（BIC）值分别为42.49±0.1 mg/mL和97.34±0.6 mg/mL。分子对接研究表明，果实和叶片挥发油的抗生物膜作用可能是由于对群体感应蛋白LasR的抑制。结果表明这些挥发油作为抗氧化剂和抗菌剂具有潜在的应用价值。
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