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叶和果实挥发油的植物化学成分分析及体外自由基清除活性研究

Phytochemical analysis and in vitro free-radical-scavenging activities of the essential oils from leaf and fruit of Melaleuca leucadendra L.

机构信息

Instituto de Investigaciones para la Industria Alimentaria, Carretera al Guatao km 3 1/2, La Habana, Cuba.

出版信息

Chem Biodivers. 2010 Sep;7(9):2281-8. doi: 10.1002/cbdv.200900162.

DOI:10.1002/cbdv.200900162
PMID:20860029
Abstract

The phytochemical profile of Melaleuca leucadendra L. leaf and fruit oils from Cuba was investigated by GC and GC/MS. Forty-one and sixty-four volatile compounds were identified and quantified, accounting for 99.2 and 99.5% of the leaf-oil and fruit-oil total composition, respectively. The main components were 1,8-cineol (43.0%), viridiflorol (24.2%), α-terpineol (7.0%), α-pinene (5.3%), and limonene (4.8%) in the leaf oil, and viridiflorol (47.6%), globulol (5.8%), guaiol (5.3%), and α-pinene (4.5%) in the fruit oil. The antioxidant capacity of these essential oils was determined by three different in vitro assays (2,2-diphenyl-1-picrylhydrazyl (DPPH) radical, thiobarbituric acid reactive species (TBARS), and 2,2'-Azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical cation), and significant activities were evidenced for all of them.

摘要

采用 GC 和 GC/MS 对古巴桃金娘科白千层叶和果实油的植物化学成分进行了研究。鉴定并定量了 41 种和 64 种挥发性化合物,分别占叶油和果油总成分的 99.2%和 99.5%。叶油中的主要成分是 1,8-桉叶素(43.0%)、桃金娘醇(24.2%)、α-松油醇(7.0%)、α-蒎烯(5.3%)和柠檬烯(4.8%),而果油中的主要成分是桃金娘醇(47.6%)、愈创木醇(5.8%)、愈创木薁醇(5.3%)和α-蒎烯(4.5%)。通过三种不同的体外测定法(2,2-二苯基-1-苦基肼基(DPPH)自由基、硫代巴比妥酸反应性物质(TBARS)和 2,2'-联氮双(3-乙基苯并噻唑啉-6-磺酸)(ABTS)自由基阳离子)测定了这些精油的抗氧化能力,所有这些精油都表现出显著的活性。

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