Arnoux B, Joseph M, Simoes M H, Tonnel A B, Duroux P, Capron A, Benveniste J
Bull Eur Physiopathol Respir. 1987 Mar-Apr;23(2):119-24.
Alveolar macrophages (AM) from control (n = 12) and atopic patients (n = 19, 12 without treatment, 4 treated with theophylline and 3 with theophylline and corticosteroid) were compared for their capacity to release mediators. AM were purified by 2 h adherence and challenged with either ubiquitous allergen, specific sensitizing allergens, anti-IgG or anti-IgE serum. The release of paf-acether, lyso paf-acether and beta-glucuronidase was measured. Paf-acether and its lyso derivative were assayed on washed rabbit platelets. Enzyme and mediator releases were obtained after specific allergenic or anti-IgE serum challenge of AM from untreated atopic patients. No release of paf-acether was detected from AM, from control or treated atopic patients after in vitro immunological challenge, whereas that of lyso paf-acether was greatly reduced in both treated groups. Release was obtained by immunological challenge of AM from control patients after passive sensitization with atopic serum. The release of mediators with bronchoconstriction activity by AM could represent an alternative causal pathway in human asthma.
对来自对照组（n = 12）和特应性患者（n = 19，其中12例未接受治疗，4例接受茶碱治疗，3例接受茶碱和皮质类固醇治疗）的肺泡巨噬细胞（AM）释放介质的能力进行了比较。通过2小时贴壁法纯化AM，并分别用常见变应原、特异性致敏变应原、抗IgG或抗IgE血清进行刺激。检测血小板活化因子乙酰酯酶（PAF - 乙酰醚）、溶血PAF - 乙酰醚和β - 葡萄糖醛酸酶的释放。在洗涤过的兔血小板上检测PAF - 乙酰醚及其溶血衍生物。从未经治疗的特应性患者的AM经特异性变应原或抗IgE血清刺激后获得酶和介质释放情况。在体外免疫刺激后，无论是对照组还是治疗组的特应性患者的AM均未检测到PAF - 乙酰醚的释放，而在两个治疗组中溶血PAF - 乙酰醚的释放均大幅减少。用特应性血清对对照组患者的AM进行被动致敏后，经免疫刺激可获得介质释放。AM释放具有支气管收缩活性的介质可能代表人类哮喘的另一种致病途径。
