从不同生境中分离的卷曲乳杆菌的表型-基因型分析：碳水化合物代谢、抗生素耐药性、细菌素、噬菌体片段以及与 CRISPR-Cas 系统的联系。

Phenotype-genotype analysis of Latilactobacills curvatus from different niches: Carbohydrate metabolism, antibiotic resistance, bacteriocin, phage fragments and linkages with CRISPR-Cas systems.

机构信息

State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China; International Joint Research Laboratory for Probiotics at Jiangnan University, Wuxi, Jiangsu 214122, China.

State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China.

出版信息

Food Res Int. 2022 Oct;160:111640. doi: 10.1016/j.foodres.2022.111640. Epub 2022 Jul 9.

DOI:10.1016/j.foodres.2022.111640

PMID:36076376

Abstract

The potential probiotic function of Latilactobacills curvatus has attracted the attention of researchers. To explore the differences in the genomes of L. curvatus, nine strains were isolated from various sources, including feces and fermented vegetables and compared with 25 strains from the NCBI database. The findings indicated that the average genome size, GC content, and CDS of L. curvatus were 1.94 MB, 41.9%, and 1825, respectively. Its core genome is associated with transcription, translation, carbohydrate transport and metabolism, and defense functions. The pan-genome of L. curvatus was in a closed state. The genetic diversity of L. curatus is mainly manifested in its ability to use carbohydrates, antibiotic resistance, bacteriocin operon, and polymeric regularly interspaced short palindromic repeats (CRISPR)-Cas for bacterial immunity. The CRISPR system of 34 strains of L. curvatus was predominantly found to be of the IIA type with a few IIC and IE types. These findings will contribute to a better understanding of this species.

摘要

卷曲乳杆菌的潜在益生菌功能引起了研究人员的关注。为了探究卷曲乳杆菌基因组的差异，从粪便和发酵蔬菜等多种来源中分离出 9 株菌株，并与 NCBI 数据库中的 25 株菌株进行比较。结果表明，卷曲乳杆菌的平均基因组大小、GC 含量和 CDS 分别为 1.94MB、41.9%和 1825。其核心基因组与转录、翻译、碳水化合物运输和代谢以及防御功能有关。卷曲乳杆菌的泛基因组处于封闭状态。卷曲乳杆菌的遗传多样性主要表现在其利用碳水化合物、抗生素耐药性、细菌素操纵子和聚合规则间隔短回文重复序列（CRISPR）-Cas 进行细菌免疫的能力上。34 株卷曲乳杆菌的 CRISPR 系统主要为 IIA 型，少数为 IIC 和 IE 型。这些发现将有助于更好地了解该物种。