不同生境来源的比较基因组分析。

Comparative Genomics Analysis of from Different Niches.

机构信息

State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China.

School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.

出版信息

Genes (Basel). 2020 Jan 14;11(1):95. doi: 10.3390/genes11010095.

The potential probiotic benefits of have received increasing attention. To investigate the genetic diversity of , comparative genomic analyses of 93 strains isolated from different niches (human and animal gut, human vagina, etc.) and eight strains of published genomes were conducted. The results showed that the core genome of mainly encoded translation and transcription, amino acid biosynthesis, sugar metabolism, and defense function while the pan-genomic curve tended to be close. The genetic diversity of mainly reflected in carbohydrate metabolism and immune/competitive-related factors, such as exopolysaccharide (EPS), enterolysin A, and clustered regularly interspaced short palindromic repeats (CRISPR)-Cas. It was worth noting that this research firstly predicted the complete EPS operon shared among . Additionally, the type IIIA CRISPR-Cas system was discovered in for the first time. This work provided new ideas for the study of this species.

已经越来越关注的潜在益生菌益处。为了研究的遗传多样性，对从不同生态位（人和动物肠道、人类阴道等）分离的 93 株和 8 株已发表基因组的菌株进行了比较基因组分析。结果表明，的核心基因组主要编码翻译和转录、氨基酸生物合成、糖代谢和防御功能，而泛基因组曲线趋于接近。的遗传多样性主要反映在碳水化合物代谢和免疫/竞争相关因子上，如胞外多糖（EPS）、肠溶素 A 和成簇规律间隔短回文重复（CRISPR）-Cas。值得注意的是，本研究首次预测了之间共享的完整 EPS 操纵子。此外，首次在中发现了 IIIA 型 CRISPR-Cas 系统。这项工作为该物种的研究提供了新的思路。