Human Nutrition Program, Department of Human Sciences, The Ohio State University, Columbus, OH 43210, USA.
Department of Biology, Miami University, Oxford, OH 45056, USA.
Food Res Int. 2022 Oct;160:111762. doi: 10.1016/j.foodres.2022.111762. Epub 2022 Jul 30.
Metabolic syndrome (MetS) is a common metatoblic disorder that leads to various adverse health outcomes such as diabetes and cardiovascular diseases (CVDs). Recent studies suggested that MetS-associated gut dysbiosis could exacerbate MetS related diseases. Green tea, a popular beverage rich in polyphenols, has showed antioxidant and anti-inflammatory effects in treating MetS through gut modulation.
This study aimed to understand the impact of green tea extract (GTE) on the composition and metabolism of gut microbiota from people with MetS.
We utilized an in-vitro human colonic model (HCM) to specifically investigate the host-free interactions between GTE and gut microbiota of MetS adults. Fresh fecal samples donated by three adults with MetS were used as gut microbe inoculum in our HCM system. 16S ribosomal RNA sequencing and liquid-chromatography mass spectrometry (LC/MS) combined with QIIME 2, Compound Discoverer 3.1 and MetaboAnalyst 4.0 based data analyses were performed to show the regulating effects of GTE treatment on gut microbial composition and their metabolism.
Our data suggested that GTE treatment in HCM system modified composition of MetS gut microbiota at genus level and led to significant microbiota metabolic profile change. Bioinformatics analysis showed relative abundance of Escherichia and Klebsiella was commonly increased while Bacteroides, Citrobacter, and Clostridium were significantly reduced. All free fatty acids detected were significantly increased in different colon sections. Lipopolysaccharide biosynthesis, methane metabolism, pentose phosphate pathway, purine metabolism, and tyrosine metabolism were regulated by GTE in MetS gut microbiota. In addition, we identified significant associations between altered microbes and microbial metabolites.
Overall, our study revealed the impact of GTE treatment on gut microbiota composition and metabolism changes in MetS microbiota in vitro, which may provide information for further mechanistic investigation of GTE in modulating gut dysbiosis in MetS.
代谢综合征（MetS）是一种常见的代谢紊乱，可导致各种不良健康后果，如糖尿病和心血管疾病（CVDs）。最近的研究表明，MetS 相关的肠道菌群失调可能会加重 MetS 相关疾病。绿茶是一种富含多酚的流行饮料，通过肠道调节显示出治疗 MetS 的抗氧化和抗炎作用。
本研究旨在了解绿茶提取物（GTE）对 MetS 患者肠道微生物组成和代谢的影响。
我们利用体外人结肠模型（HCM）专门研究 GTE 与 MetS 成年人肠道微生物群之间的无宿主相互作用。使用来自三名 MetS 成年人的新鲜粪便样本作为我们 HCM 系统中的肠道微生物接种物。我们进行了 16S 核糖体 RNA 测序和液相色谱-质谱（LC/MS）联合 QIIME 2、Compound Discoverer 3.1 和 MetaboAnalyst 4.0 基于数据分析，以显示 GTE 处理对肠道微生物组成及其代谢的调节作用。
我们的数据表明，GTE 处理在 HCM 系统中改变了 MetS 肠道微生物群在属水平上的组成，并导致了显著的微生物代谢谱变化。生物信息学分析显示，大肠杆菌和克雷伯氏菌的相对丰度普遍增加，而拟杆菌、柠檬酸杆菌和梭菌的相对丰度显著降低。不同结肠段检测到的所有游离脂肪酸均显著增加。GTE 在 MetS 肠道微生物群中调节了脂多糖生物合成、甲烷代谢、戊糖磷酸途径、嘌呤代谢和酪氨酸代谢。此外，我们还发现了改变的微生物与微生物代谢物之间的显著关联。
总的来说，我们的研究揭示了 GTE 处理对 MetS 微生物体外肠道微生物群组成和代谢变化的影响，这可能为进一步研究 GTE 调节 MetS 肠道菌群失调的机制提供信息。
