Human Nutrition Program, Department of Human Sciences, The Ohio State University, Columbus, OH 43210, USA.
Department of Biology, Miami University, Oxford, OH 45056, USA.
J Chromatogr B Analyt Technol Biomed Life Sci. 2022 Jan 1;1188:123027. doi: 10.1016/j.jchromb.2021.123027. Epub 2021 Nov 17.
Human gut microbiota is critical for human health, as their dysbiosis could lead to various diseases such as irritable bowel syndrome and obesity. Black raspberry (BRB) has been increasingly studied recently for its impact on gut microbiota as a rich source of phytochemicals (e.g., anthocyanin). To investigate the effect of BRB extract on the gut microbiota composition and their metabolism, an in-vitro human colonic model (HCM) was utilized to study the direct interaction between BRB and gut microbiome. Conditions (e.g., pH, temperature, anaerobic environment) in HCM were closely monitored and maintained to simulate the human intestinal system. Fresh fecal samples donated by three young healthy volunteers were used for gut microbiota inoculation in the HCM. 16S ribosomal DNA sequencing and liquid-chromatography mass spectrometry (LC/MS) based metabolomics were performed to study the impact of BRB on gut microbiota characteristics and their metabolism (fatty acids, polar metabolites, and phenolic compounds). Our data suggested that BRB intervention modulated gut microbiota at the genus level in different HCM sections mimicing ascending, transverse, and descending colons. Relative abundance of Enterococcus was commonly decreased in all colon sections, while modulations of other bacteria genera were mostly location-dependent. Meanwhile, significant changes in the metabolic profile of gut microbiota related to fatty acids, endogenous polar metabolites, and phenolic compounds were detected, in which arginine and proline metabolism, lysine degradation, and aminoacyl-tRNA biosynthesis were mostly regulated. Moreover, we identified several significant associations between altered microbial populations and changes in microbial metabolites. In summary, our study revealed the impact of BRB intervention on gut microbiota composition and metabolism change, which may exert physiological change to host metabolism and host health.
人类肠道微生物群对人类健康至关重要，因为它们的失调可能导致各种疾病，如肠易激综合征和肥胖症。黑覆盆子（BRB）由于其作为植物化学物质（如花青素）的丰富来源对肠道微生物群的影响而受到越来越多的研究。为了研究 BRB 提取物对肠道微生物群组成及其代谢的影响，利用体外人类结肠模型（HCM）研究 BRB 与肠道微生物群之间的直接相互作用。密切监测和维持 HCM 中的条件（例如 pH、温度、厌氧环境）以模拟人体肠道系统。使用来自三名年轻健康志愿者的新鲜粪便样本在 HCM 中进行肠道微生物群接种。进行 16S 核糖体 DNA 测序和基于液相色谱-质谱（LC/MS）的代谢组学研究，以研究 BRB 对肠道微生物群特征及其代谢（脂肪酸、极性代谢物和酚类化合物）的影响。我们的数据表明，BRB 干预在模拟升结肠、横结肠和降结肠的不同 HCM 节段调节肠道微生物群在属水平上。在所有结肠节段中，肠球菌的相对丰度通常降低，而其他细菌属的调节大多依赖于位置。同时，还检测到与肠道微生物群相关的脂肪酸、内源性极性代谢物和酚类化合物的代谢谱发生显著变化，其中精氨酸和脯氨酸代谢、赖氨酸降解和氨基酸酰-tRNA 生物合成大多受到调节。此外，我们还发现了一些改变的微生物种群与微生物代谢物变化之间的显著关联。总之，我们的研究揭示了 BRB 干预对肠道微生物群组成和代谢变化的影响，这可能会对宿主代谢和宿主健康产生生理变化。
