一种新的技术，通过共聚体设备分离富含血小板的血浆 - 无需离心过程。
A new technique for separating platelet-rich plasma by a copolymer device － without a centrifugation process.
机构信息
Department of Neurosurgery, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung 83301, Taiwan.
Department of Chemical and Materials Engineering, National University of Kaohsiung, Kaohsiung University Rd., Nan-Tsu District, Kaohsiung 811, Taiwan.
出版信息
Biomed Pharmacother. 2022 Sep;153:113481. doi: 10.1016/j.biopha.2022.113481. Epub 2022 Aug 2.
BACKGROUND
Platelet-rich plasma (PRP) is beneficial for clinical applications in various medical fields. Although commercial PRP preparation kits are already available in the market, most of these kits employ centrifugation.
METHODS
We used a new cationic copolymer coating on a polyurethane (PU) sponge to promote platelet separation from the blood. This copolymer showed no cytotoxicity against cell viability or hemolysis. We further evaluated the efficiency of the new PRP preparation device by comparing it with that of a commercially available kit (RegenKit-THT).
RESULTS
We demonstrated that PRP obtained using copolymer device contains high concentrations of platelets and angiogenic growth factors (epidermal growth factor, vascular endothelial growth factor-A, growth differentiation factor 2, and interleukin-8). The separated PRP also displayed beneficial effects on cell migration, angiogenesis, and matrix metalloproteinase gene expression.
CONCLUSION
Based on these results, we developed a cationic copolymer-coated PU sponge as a PRP preparation device without the need for any centrifugation.
背景
富含血小板的血浆（PRP）在各个医学领域的临床应用中都有好处。尽管市场上已经有商用的 PRP 制备试剂盒，但这些试剂盒大多采用离心法。
方法
我们在聚氨酯（PU）海绵上使用了一种新的阳离子共聚物涂层来促进血小板从血液中分离。这种共聚物对细胞活力或溶血没有细胞毒性。我们通过与市售试剂盒（RegenKit-THT）进行比较，进一步评估了新的 PRP 制备装置的效率。
结果
我们证明了使用共聚物装置获得的 PRP 含有高浓度的血小板和血管生成生长因子（表皮生长因子、血管内皮生长因子-A、生长分化因子 2 和白细胞介素-8）。分离的 PRP 还显示出对细胞迁移、血管生成和基质金属蛋白酶基因表达的有益作用。
结论
基于这些结果，我们开发了一种阳离子共聚物涂覆的 PU 海绵作为 PRP 制备装置，无需任何离心。
Zotero 插件