Department of Home Sciences, Faculty of Agriculture, Federal University, Gashua, P.M.B.1005 Gashua, Yobe State, Nigeria; Department of Food Science and Technology, Federal University of Agriculture, Makurdi, Benue State, Nigeria.
Department of Food Science and Technology, Federal University of Agriculture, Makurdi, Benue State, Nigeria.
Biomed Pharmacother. 2022 Sep;153:113510. doi: 10.1016/j.biopha.2022.113510. Epub 2022 Aug 5.
Balanites aeqyptiaca (BA) seeds were toasted at 70 °C, milled and the oil expelled to resolve to meal which were defatted to resolve to defatted balanites aeqyptiaca (BA) protein meal and (BA) protein concentrate respectively. These were subjected to analysis using standard methods. There exist marked trend between defatted balanites aeqyptiaca protein meal, protein concentrate and incidences of diabetes. This work investigated the anti- diabetic effects of balanites aeqyptiaca defatted protein meal and concentrate supplemented diets in streptozotocin (STZ)-induced diabetic rats. The rats were fattened for two weeks with high fat diet (HFD) to introduce Hyperglycemia and then made diabetic by intraperitoneal administration of STZ (35 mg/kg body weight) and fed diets containing 5 % defatted balanites aeqyptiaca protein meal (DAPM) and 5 % balanites aeqyptiaca protein concentrate (APC) for 14 days. The effect of the diet on blood glucose, serum glutathione peroxidase (GPx), glutathione transferase (GSH), thiobarbituric acid reactive species (TBARS), α-amylase and intestinal α-glucosidase activities were investigated. There was marked increase in the blood glucose, TBARS, pancreatic α-amylase and intestinal α-glycosidase with corresponding decrease in serum GPx and GSH contents in diabetic rats control groups. These trends were however, reversed in diabetic rats fed diet supplemented with the balanites aeqyptiaca protein meals for 14 days. The meals from defatted and protein concentrate inhibit α-amylase and α-glycosidase inhibitory activity in vivo. Thus, the anti-diabetes properties of the defatted meal and protein concentrate may be attributed to the influence of its constituent phytochemicals on starch digestion as well as endogenous enzymes activities. The study revealed that defatted aduwa meal and proteins concnentrate demonstrated potentials used as functional ingredients in food materials and could also increase income access of low resource populace.
刺槐豆种子在 70°C 下烘烤，磨碎，挤出油，得到豆粕，然后将豆粕脱脂，分别得到脱脂刺槐豆蛋白粕和（BA）蛋白浓缩物。这些分别用标准方法进行分析。脱脂刺槐豆蛋白粕、蛋白浓缩物和糖尿病发病率之间存在显著趋势。本工作研究了刺槐豆脱脂蛋白粕和浓缩物补充饮食对链脲佐菌素（STZ）诱导的糖尿病大鼠的抗糖尿病作用。大鼠用高脂肪饮食（HFD）饲养两周以引起高血糖，然后用腹腔注射 STZ（35mg/kg 体重）诱导糖尿病，并喂食含有 5%脱脂刺槐豆蛋白粕（DAPM）和 5%刺槐豆蛋白浓缩物（APC）的饮食 14 天。研究了饮食对血糖、血清谷胱甘肽过氧化物酶（GPx）、谷胱甘肽转移酶（GSH）、硫代巴比妥酸反应物（TBARS）、α-淀粉酶和肠α-葡萄糖苷酶活性的影响。糖尿病大鼠对照组的血糖、TBARS、胰腺α-淀粉酶和肠α-葡萄糖苷酶显著升高，血清 GPx 和 GSH 含量相应降低。然而，在喂食含刺槐豆蛋白餐 14 天的糖尿病大鼠中，这些趋势得到了逆转。脱脂和蛋白浓缩物中的膳食抑制了体内α-淀粉酶和α-葡萄糖苷酶的活性。因此，脱脂餐和蛋白浓缩物的抗糖尿病特性可能归因于其成分植物化学物质对淀粉消化以及内源性酶活性的影响。该研究表明，脱脂刺槐豆粉和蛋白浓缩物具有作为功能性食品成分的潜力，可增加低资源人群的收入。
