Ajiboye Adekunle O, Shodehinde Sidiqat A
West Virginia University, Animal and Nutritional Sciences Davis College of Agriculture, Natural Resources & Design, Morgantown, West Virginia, USA.
Department of Biochemistry, Faculty of Science, Adekunle Ajasin University, Akungba Akoko, Ondo State, Nigeria.
J Food Biochem. 2022 Dec;46(12):e14431. doi: 10.1111/jfbc.14431. Epub 2022 Oct 6.
The ameliorating effect and antidiabetic properties of diets augmented with boiled unripe plantain (20%-40%) in high fat fed/low dose of streptozotocin induced diabetic rats in comparison with the administration of acarbose were evaluated in this study using standard methods. High fat fed/low dose of streptozotocin (25 mg/kg body weight) was given to twenty-five male Wistar rats to induce diabetes leaving out 5 normal rats to serve as control. The animals were separated into five with six rats in each group and the experiment continued for 14 days. Investigations on the blood glucose concentration, enzymes (α-amylase, α-glucosidase, angiotensin I converting enzyme), thiobarbituric reaction substance (TBARS), High-density lipoprotein-cholesterol (HDL-c), and antioxidant status were determined. The findings revealed a rise in blood glucose level and the activities of α-amylase, α-glucosidase, angiotensin I converting enzyme, thiobarbituric reaction substance (TBARS) in untreated diabetic rats in group II while a reverse was observed in diabetic rats (Group IV and V) on exposure to diets augmented with boiled unripe plantain. The obtained overall results in diet treated groups are similar to that of acarbose treated groups. The untreated diabetic rats (Group II) exhibited contrary results of the biochemical assays. This finding showed that boiled unripe plantain can provide the therapeutic measures that needed to be further explored as possible future economic means of managing diabetes in developing nations. PRACTICAL APPLICATIONS: As diabetes has been implicated to disrupt various pathways involved in the metabolism of macromolecules, there are proposed adoptive methods of preventing them among which is the inhibition of starch hydrolyzing enzymes, increasing the enzymatic antioxidant status and prevention of lipid peroxidation, Plantain by-product which is known as an inexpensive food can be prepared to manage the condition of diabetes in patients. Our former in vitro findings have revealed the bioactive contents of unripe plantain product which has been further explored in vivo to experiment is nutritional benefits. The study therefore proposes that unripe plantains, when boiled, can provide the necessary natural therapeutic measures to be considered as a potential economic means of managing diabetes in underdeveloped countries.
本研究采用标准方法，评估了用煮熟的未成熟芭蕉（20%-40%）强化的饮食对高脂喂养/低剂量链脲佐菌素诱导的糖尿病大鼠的改善作用和抗糖尿病特性，并与阿卡波糖的给药效果进行了比较。给25只雄性Wistar大鼠喂食高脂/低剂量链脲佐菌素（25mg/kg体重）以诱导糖尿病，留下5只正常大鼠作为对照。将动物分成五组，每组6只大鼠，实验持续14天。测定了血糖浓度、酶（α-淀粉酶、α-葡萄糖苷酶、血管紧张素I转换酶）、硫代巴比妥酸反应物质（TBARS）、高密度脂蛋白胆固醇（HDL-c）和抗氧化状态。研究结果显示，未治疗的II组糖尿病大鼠的血糖水平以及α-淀粉酶、α-葡萄糖苷酶、血管紧张素I转换酶、硫代巴比妥酸反应物质（TBARS）的活性有所升高，而在喂食煮熟的未成熟芭蕉强化饮食的糖尿病大鼠（IV组和V组）中观察到相反的情况。饮食治疗组获得的总体结果与阿卡波糖治疗组相似。未治疗的糖尿病大鼠（II组）的生化检测结果则相反。这一发现表明，煮熟的未成熟芭蕉可以提供所需的治疗措施，作为发展中国家未来管理糖尿病的潜在经济手段，有待进一步探索。实际应用：由于糖尿病被认为会扰乱参与大分子代谢的各种途径，因此提出了多种预防方法，其中包括抑制淀粉水解酶、提高酶促抗氧化状态和预防脂质过氧化，已知芭蕉副产品是一种廉价食物，可以制备用于管理糖尿病患者的病情。我们之前的体外研究结果揭示了未成熟芭蕉产品的生物活性成分，并在体内进一步探索了其营养益处。因此，该研究提出，煮熟的未成熟芭蕉可以提供必要的自然治疗措施，被视为欠发达国家管理糖尿病的潜在经济手段。
