Iseppi Ramona, Zurlini Chiara, Cigognini Ilaria Maria, Cannavacciuolo Mariarosaria, Sabia Carla, Messi Patrizia
Department of Life Sciences, University of Modena and Reggio Emilia, Via G. Campi 287, 41125 Modena, Italy.
SSICA-Stazione Sperimentale per l'Industria delle Conserve Alimentari, Viale F. Tanara 31/A, 43121 Parma, Italy.
Foods. 2022 Aug 30;11(17):2632. doi: 10.3390/foods11172632.
To meet consumer requirements for high quality food free of chemical additives, according to the principles of sustainability and respect for the environment, new “green” packaging solutions have been explored. The antibacterial activity of edible bioactive films and coatings, based on biomolecules from processing by-products and biomasses, added with the bacteriocin producer Lactobacillus kefiri MM5, has been determined in vegetables against L. monocytogenes NCTC 10888 (i) “in vitro” by a modified agar diffusion assay and (ii) “on food” during storage of artificially contaminated raw vegetable samples, after application of active films and coatings. Both polysaccharides-based and proteins-based films and coatings showed excellent antilisterial activity, especially at 10 and 20 days. Protein-based films displayed a strong activity against L. monocytogenes in carrots and zucchini samples (p < 0.0001). After 10 days, both polysaccharide-based and protein-based films demonstrated more enhanced activity than coatings towards the pathogen. These edible active packagings containing live probiotics can be used both to preserve the safety of fresh vegetables and to deliver a beneficial probiotic bacterial strain. The edible ingredients used for the formulation of both films and coatings are easily available, at low cost and environmental impact.
为满足消费者对无化学添加剂的高品质食品的需求，根据可持续发展和尊重环境的原则，人们探索了新的“绿色”包装解决方案。基于加工副产品和生物质中的生物分子，并添加产细菌素的开菲尔乳杆菌MM5的可食用生物活性薄膜和涂层，在蔬菜中针对单核细胞增生李斯特菌NCTC 10888的抗菌活性已通过以下方式测定：(i) 通过改良琼脂扩散法在“体外”测定；(ii) 在人工污染的生蔬菜样品储存期间，在涂抹活性薄膜和涂层后在“食品上”测定。基于多糖和基于蛋白质的薄膜和涂层均表现出优异的抗李斯特菌活性，尤其是在第10天和第20天。基于蛋白质的薄膜在胡萝卜和西葫芦样品中对单核细胞增生李斯特菌表现出较强的活性（p < 0.0001）。10天后，基于多糖和基于蛋白质的薄膜对病原体的活性均比涂层更强。这些含有活益生菌的可食用活性包装可用于保持新鲜蔬菜的安全性，并提供有益的益生菌菌株。用于制备薄膜和涂层的可食用成分易于获得，成本低且对环境影响小。
