Dipartimento di Scienze Chimiche Biologiche Farmaceutiche ed Ambientali (CHIBIOFARAM), Università degli Studi di Messina, 98166 Messina, Italy.
School of Natural and Environmental Sciences, Newcastle University, Newcastle upon Tyne NE1 7RU, UK.
Int J Mol Sci. 2022 Aug 23;23(17):9542. doi: 10.3390/ijms23179542.
In this paper we describe the synthesis of a novel bichromophoric system in which an efficient photoinduced intercomponent energy transfer process is active. The dyad consists of one subunit of curcumin and one of BODIPY and is able to emit in the far-red region, offering a large Stokes shift, capable of limiting light scattering processes for applications in microscopy. The system has been encapsulated in MCM-41 nanoparticles with dimensions between 50 and 80 nm. Both the molecular dyad and individual subunits were tested with different cell lines to study their effective applicability in bioimaging. MCM-41 nanoparticles showed no reduction in cell viability, indicating their biocompatibility and bio-inertness and making them capable of delivering organic molecules even in aqueous-based formulations, avoiding the toxicity of organic solvents. Encapsulation in the porous silica structure directed the location of the bichromophoric system within cytoplasm, while the dyad alone stains the nucleus of the hFOB cell line.
在本文中，我们描述了一种新型双生色体系的合成，其中存在有效的光诱导分子间能量转移过程。该偶联物由姜黄素的一个亚基和 BODIPY 的一个亚基组成，能够在远红区发射，提供大的斯托克斯位移，能够限制用于显微镜应用的光散射过程。该体系已被封装在尺寸为 50 至 80nm 的 MCM-41 纳米颗粒中。对分子偶联物和各个亚基进行了不同细胞系的测试，以研究它们在生物成像中的有效适用性。MCM-41 纳米颗粒没有降低细胞活力，表明它们具有生物相容性和生物惰性，并且能够即使在基于水的制剂中输送有机分子，避免有机溶剂的毒性。在多孔硅结构中的封装将双生色体系的位置导向细胞质内，而偶联物本身则染色 hFOB 细胞系的细胞核。
