Advanced Magnetic Materials Research Center, School of Metallurgy and Materials, College of Engineering, University of Tehran, Tehran 11155-4563, Iran.
Department of Life Science Engineering, Faculty of New Science and Technologies, University of Tehran, Tehran 14179-35840, Iran.
Int J Mol Sci. 2022 Aug 25;23(17):9657. doi: 10.3390/ijms23179657.
In this study, the protein stability of hen egg-white lysozymes (HEWL) by FeO and FeO-coated trehalose (FeO@Tre) magnetic nanoparticles (NPs) is investigated. For this purpose, the co-precipitation method was used to synthesize magnetic NPs. The synthesized NPs were characterized by XRD, FT-IR spectroscopy, FE-SEM, and VSM analysis. In addition, the stability of HEWLs exposed to different NP concentrations in the range of 0.001-0.1 mg mL was investigated by circular dichroism (CD) spectroscopy, fluorescence, and UV-Vis analysis. Based on the results, in the NP concentration range of 0.001-0.04 mg mL the protein structure is more stable, and this range was identified as the range of kosmotropic concentration. The helicity was measured at two concentration points of 0.02 and 0.1 mg mL. According to the results, the α-helix at 0.02 mg mL of FeO and FeO@Tre was increased from 35.5% for native protein to 37.7% and 38.7%, respectively. The helicity decreased to 36.1% and 37.4%, respectively, with increasing the concentration of FeO and FeO@Tre to 0.1 mg mL. The formation of hydrated water shells around protein molecules occurred by using FeO@Tre NPs. Hence, it can be concluded that the trehalose as a functional group along with magnetic NPs can improve the stability of proteins in biological environments.
在这项研究中，通过 FeO 和 FeO 包覆海藻糖（FeO@Tre）磁性纳米颗粒（NPs）研究了鸡卵清溶菌酶（HEWL）的蛋白质稳定性。为此，采用共沉淀法合成了磁性 NPs。通过 XRD、FT-IR 光谱、FE-SEM 和 VSM 分析对合成的 NPs 进行了表征。此外，通过圆二色性（CD）光谱、荧光和 UV-Vis 分析研究了 HEWLs 在 0.001-0.1 mg mL 范围内不同 NP 浓度下的稳定性。基于结果，在 0.001-0.04 mg mL 的 NP 浓度范围内，蛋白质结构更稳定，该范围被确定为亲水性浓度范围。在 0.02 和 0.1 mg mL 的两个浓度点测量了螺旋度。结果表明，在 0.02 mg mL 的 FeO 和 FeO@Tre 中，α-螺旋从天然蛋白的 35.5%增加到 37.7%和 38.7%。当 FeO 和 FeO@Tre 的浓度增加到 0.1 mg mL 时，螺旋度分别降低到 36.1%和 37.4%。通过 FeO@Tre NPs 在蛋白质分子周围形成水合水壳。因此，可以得出结论，海藻糖作为一种功能基团与磁性 NPs 一起可以提高蛋白质在生物环境中的稳定性。
Zotero 插件
