猪流行性腹泻病毒刺突蛋白上新的中和表位。
A New Neutralization Epitope in the Spike Protein of Porcine Epidemic Diarrhea Virus.
机构信息
State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of the Chinese Academy of Agricultural Sciences, 678 Haping Road, Xiangfang District, Harbin 150069, China.
出版信息
Int J Mol Sci. 2022 Aug 26;23(17):9674. doi: 10.3390/ijms23179674.
Porcine epidemic diarrhea virus (PEDV) infects piglets and causes serious diarrhea as well as vomiting, dehydration, and death. The trimeric S protein plays a crucial role in the induction of neutralizing antibodies, and many neutralizing monoclonal antibodies (mAbs) against PEDV S protein have been developed. However, these mAbs exclusively target the S1 protein. In this study, we obtained a neutralizing mAb, 5F7, against the S2 protein of PEDV, and this mAb could neutralize new variant genotype 2 PEDV strains (LNCT2), as well as a genotype 1 PEDV strain (CV777), in vitro. The core sequence of the epitope was found in amino acid sequence 1261 aa~1337 aa. These findings confirm that the S2 protein possessed neutralizing epitopes and provided knowledge to aid further research on this virus.
猪流行性腹泻病毒（PEDV）感染仔猪，引起严重腹泻、呕吐、脱水和死亡。三聚体 S 蛋白在诱导中和抗体中起关键作用，已经开发出许多针对 PEDV S 蛋白的中和单克隆抗体（mAb）。然而，这些 mAb 专门针对 S1 蛋白。在这项研究中，我们获得了针对 PEDV S2 蛋白的中和 mAb 5F7，该 mAb 可以中和新型变异基因型 2 PEDV 株（LNCT2）以及基因型 1 PEDV 株（CV777），在体外。该表位的核心序列位于氨基酸序列 1261 aa~1337 aa。这些发现证实 S2 蛋白具有中和表位，为进一步研究该病毒提供了知识。
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