State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of the Chinese Academy of Agricultural Sciences, 678 Haping Road, Xiangfang District, Harbin 150069, China.
Int J Mol Sci. 2022 Aug 26;23(17):9674. doi: 10.3390/ijms23179674.
Porcine epidemic diarrhea virus (PEDV) infects piglets and causes serious diarrhea as well as vomiting, dehydration, and death. The trimeric S protein plays a crucial role in the induction of neutralizing antibodies, and many neutralizing monoclonal antibodies (mAbs) against PEDV S protein have been developed. However, these mAbs exclusively target the S1 protein. In this study, we obtained a neutralizing mAb, 5F7, against the S2 protein of PEDV, and this mAb could neutralize new variant genotype 2 PEDV strains (LNCT2), as well as a genotype 1 PEDV strain (CV777), in vitro. The core sequence of the epitope was found in amino acid sequence 1261 aa~1337 aa. These findings confirm that the S2 protein possessed neutralizing epitopes and provided knowledge to aid further research on this virus.
猪流行性腹泻病毒（PEDV）感染仔猪，引起严重腹泻、呕吐、脱水和死亡。三聚体 S 蛋白在诱导中和抗体中起关键作用，已经开发出许多针对 PEDV S 蛋白的中和单克隆抗体（mAb）。然而，这些 mAb 专门针对 S1 蛋白。在这项研究中，我们获得了针对 PEDV S2 蛋白的中和 mAb 5F7，该 mAb 可以中和新型变异基因型 2 PEDV 株（LNCT2）以及基因型 1 PEDV 株（CV777），在体外。该表位的核心序列位于氨基酸序列 1261 aa~1337 aa。这些发现证实 S2 蛋白具有中和表位，为进一步研究该病毒提供了知识。
