Fu Fang, Li Lin, Shan Lingling, Yang Beibei, Shi Hongyan, Zhang Jiaoer, Wang Hongfeng, Feng Li, Liu Pinghuang
State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China.
Weike Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China.
Vet Microbiol. 2017 Jun;205:99-105. doi: 10.1016/j.vetmic.2017.05.013. Epub 2017 May 21.
Porcine epidemic diarrhea (PED), caused by an alpha coronavirus, is a highly contagious disease and causes high morbidity and mortality in suckling piglets. Isolating PEDV neutralizing antibodies from porcine B cells is critical to elucidate the development of PEDV neutralizing antibodies and the protective mechanism of PEDV infection. Here, we described the isolation of a PEDV-neutralizing antibody from the B cell of a vaccinated pig. The antibody, named PC10, was demonstrated to target the conformational epitope of PEDV spike protein, specifically bind to the infected cells of PEDV genogroup 1 and 2 strains, and potently neutralize PEDV infection. PC10 neutralized PEDV infection through interfering with the viral life stages after cellular attachment instead of blocking the attachment of PEDV to cells. These results suggest that PC10 could be a promising candidate for passive protection and inform PEDV vaccine design because of its specificity and substantial neutralization potency.
猪流行性腹泻（PED）由一种α冠状病毒引起，是一种高度传染性疾病，可导致哺乳仔猪的高发病率和死亡率。从猪B细胞中分离猪流行性腹泻病毒（PEDV）中和抗体对于阐明PEDV中和抗体的产生及PEDV感染的保护机制至关重要。在此，我们描述了从一头接种疫苗的猪的B细胞中分离出一种PEDV中和抗体的过程。该抗体名为PC10，被证明靶向PEDV刺突蛋白的构象表位，能特异性结合PEDV基因组1型和2型毒株的感染细胞，并有效中和PEDV感染。PC10通过干扰病毒在细胞附着后的生命阶段而非阻断PEDV与细胞的附着来中和PEDV感染。这些结果表明，由于其特异性和强大的中和效力，PC10可能是被动保护的一个有前景的候选者，并为PEDV疫苗设计提供参考。
