Division of Medical Pharmacology, Department of Pharmacology, School of Medicine, Showa University, Tokyo 142-8555, Japan.
Division of Neurology, Department of Internal Medicine, School of Medicine, Showa University, Tokyo 142-8555, Japan.
Int J Mol Sci. 2022 Aug 26;23(17):9685. doi: 10.3390/ijms23179685.
Alzheimer's disease (AD) is a neurodegenerative disease that leads to progressive cognitive decline. Several effective natural components have been identified for the treatment of AD. However, it is difficult to obtain conclusive evidence on the safety and effectiveness of natural components, because a variety of factors are associated with the progression of AD pathology. We hypothesized that a therapeutic effect could be achieved by combining multiple ingredients with different efficacies. The purpose of this study was thus to evaluate a combination treatment of curcumin (Cur) and ferulic acid (FA) for amyloid-β (Aβ)-induced neuronal cytotoxicity. The effect of Cur or FA on Aβ aggregation using thioflavin T assay was confirmed to be inhibited in a concentration-dependent manner by Cur single or Cur + FA combination treatment. The effects of Cur + FA on the cytotoxicity of human neuroblastoma (SH-SY5Y) cells induced by Aβ exposure were an increase in cell viability, a decrease in ROS and mitochondrial ROS, and repair of membrane damage. Combination treatment showed an overall higher protective effect than treatment with Cur or FA alone. These results suggest that the combined action mechanisms of Cur and FA may be effective in preventing and suppressing the progression of AD.
阿尔茨海默病（AD）是一种神经退行性疾病，可导致进行性认知能力下降。已经确定了几种有效的天然成分来治疗 AD。然而，由于与 AD 病理进展相关的多种因素，很难获得关于天然成分安全性和有效性的明确证据。我们假设通过将具有不同疗效的多种成分组合使用，可以达到治疗效果。因此，本研究旨在评估姜黄素（Cur）和阿魏酸（FA）联合治疗对淀粉样蛋白-β（Aβ）诱导的神经元细胞毒性的影响。用噻唑蓝 T 试验证实，Cur 单药或 Cur+FA 联合治疗可浓度依赖性地抑制 Aβ聚集。Cur+FA 对 Aβ暴露诱导的人神经母细胞瘤（SH-SY5Y）细胞的细胞毒性的作用表现为细胞活力增加、ROS 和线粒体 ROS 减少以及膜损伤修复。联合治疗的整体保护效果高于单独使用 Cur 或 FA。这些结果表明，Cur 和 FA 的联合作用机制可能有效预防和抑制 AD 的进展。
