College of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China.
School of Medicine, Southern University of Science and Technology, Shenzhen 518055, China.
Int J Mol Sci. 2022 Aug 28;23(17):9754. doi: 10.3390/ijms23179754.
Defensins play an important role in fighting bacteria, and are a good candidate for bactericidal agents. However, the function and mechanism of defensins in regulating host responses against bacteria is unclear. In this study, transcriptome analysis was used to study the comprehensive functions of pBD2 in IPEC-J2 cells against . In total, 230 differentially expressed genes (DEGs) were identified in IPEC-J2 cells between the control and groups, and were found by KEGG analysis to be involved in many signaling pathways related to immunity. Furthermore, 812 DEGs were observed between and +pBD2 groups, involved in the ribosome, oxidative phosphorylation, and certain disease pathways. Among these, 94 overlapping DEGs were in the two DEG groups, and 85 DEGs were reverse expression, which is involved in microRNA in cancer, while PTEN and CDC6 were key genes according to PPI net analysis. The results of qRT-PCR verified those of RNA-seq. The results indicated that pBD2 plays an important role against by acting on the genes related to immune response, cell cycle, ribosomes, oxidative phosphorylation, etc. The results provide new insights into the potential function and mechanism of pBD2 against . Meanwhile, this study provides a certain theoretical basis for research and the development of novel peptide drugs.
防御素在抵抗细菌方面发挥着重要作用，是一种很好的杀菌候选物。然而，防御素在调节宿主对细菌的反应方面的功能和机制尚不清楚。在本研究中，通过转录组分析研究了 pBD2 在 IPEC-J2 细胞中针对 的综合功能。在对照和 组的 IPEC-J2 细胞之间，共鉴定出 230 个差异表达基因（DEGs），通过 KEGG 分析发现它们参与了许多与免疫相关的信号通路。此外，在 和 +pBD2 组之间观察到 812 个 DEGs，涉及核糖体、氧化磷酸化和某些疾病途径。其中，在这两个 DEG 组中有 94 个重叠的 DEGs，85 个 DEGs 的表达呈反向，涉及癌症中的 microRNA，而根据 PPI 网络分析，PTEN 和 CDC6 是关键基因。qRT-PCR 的结果验证了 RNA-seq 的结果。结果表明，pBD2 通过作用于与免疫反应、细胞周期、核糖体、氧化磷酸化等相关的基因，在抵抗 方面发挥着重要作用。该研究结果为 pBD2 抵抗 的潜在功能和机制提供了新的见解。同时，本研究为新型肽类药物的研究和开发提供了一定的理论基础。
