Institute of Higher Nervous Activity and Neurophysiology, Russian Academy of Sciences, 117485 Moscow, Russia.
Int J Mol Sci. 2022 Sep 1;23(17):9948. doi: 10.3390/ijms23179948.
We performed RNA sequencing of the dorsal and ventral parts of the hippocampus and compared it with previously published data to determine the differences in the dorsoventral gradients of gene expression that may result from biological or technical variability. Our data suggest that the dorsal and ventral parts of the hippocampus differ in the expression of genes related to signaling pathways mediated by classical neurotransmitters (glutamate, GABA, monoamines, etc.) as well as peptide and Wnt ligands. These hippocampal parts also diverge in the expression of axon-guiding molecules (both receptors and ligands) and splice isoforms of genes associated with intercellular signaling and cell adhesion. Furthermore, analysis of differential expressions of genes specific for astrocytes, microglia, oligodendrocytes, and vascular cells suggests that non-neuronal cells may also differ in the characteristics between hippocampal parts. Analysis of expression of transposable elements showed that depletion of ribosomal RNA strongly increased the representation of transposable elements in the RNA libraries and helped to detect a weak predominance of expression of these elements in the ventral hippocampus. Our data revealed new molecular dimensions of functional differences between the dorsal and ventral hippocampus and points to possible cascades that may be involved in the longitudinal organization of the hippocampus.
我们对海马体的背侧和腹侧部分进行了 RNA 测序，并与之前发表的数据进行了比较，以确定可能由于生物学或技术变异性导致的基因表达背腹梯度差异。我们的数据表明，海马体的背侧和腹侧部分在与经典神经递质（谷氨酸、GABA、单胺等）介导的信号通路以及肽和 Wnt 配体相关的基因表达上存在差异。这些海马体部分在轴突导向分子（受体和配体）以及与细胞间信号传递和细胞黏附相关的基因的剪接异构体的表达上也存在差异。此外，对特定于星形胶质细胞、小胶质细胞、少突胶质细胞和血管细胞的基因的差异表达分析表明，非神经元细胞在海马体部分之间的特征上也可能存在差异。转座元件表达分析表明，核糖体 RNA 的耗竭强烈增加了 RNA 文库中转座元件的代表性，并有助于检测到这些元件在腹侧海马体中表达的微弱优势。我们的数据揭示了背侧和腹侧海马体之间功能差异的新分子维度，并指出可能涉及海马体纵向组织的级联反应。
