Department of Food and Nutrition, Andong National University, Andong 36729, Korea.
Department of Molecular Medicine, Cell & Matrix Research Institute, School of Medicine, Kyungpook National University, Daegu 41944, Korea.
Int J Mol Sci. 2022 Sep 1;23(17):9955. doi: 10.3390/ijms23179955.
Hemp ( L.) is used for medicinal purposes owing to its anti-inflammatory and antioxidant activities. We evaluated the protective effect of nanovesicles isolated from hemp plant parts (root, seed, hemp sprout, and leaf) in dextran sulfate sodium (DSS)-induced colitis in mice. The particle sizes of root-derived nanovesicles (RNVs), seed-derived nanovesicles (SNVs), hemp sprout-derived nanovesicles (HSNVs), and leaf-derived nanovesicles (LNVs) were within the range of 100-200 nm as measured by nanoparticle tracking analysis. Acute colitis was induced in C57BL/N mice by 5% DSS in water provided for 7 days. RNVs were administered orally once a day, leading to the recovery of both the small intestine and colon lengths. RNVs, SNVs, and HSNVs restored the tight (ZO-1, claudin-4, occludin) and adherent junctions (E-cadherin and α-tubulin) in DSS-induced small intestine and colon injury. Additionally, RNVs markedly reduced NF-κB activation and oxidative stress proteins in DSS-induced small intestine and colon injury. Tight junction protein expression and epithelial cell permeability were elevated in RNV-, SNV-, and HSNV-treated T84 colon cells exposed to 2% DSS. Interestedly, RNVs, SNVs, HSNVs, and LNVs reduced ALT activity and liver regeneration marker proteins in DSS-induced liver injury. These results showed for the first time that hemp-derived nanovesicles (HNVs) exhibited a protective effect on DSS-induced gut leaky and liver injury through the gut-liver axis by inhibiting oxidative stress marker proteins.
大麻（L.）因其具有抗炎和抗氧化活性而被用于药用目的。我们评估了从大麻植物部分（根、种子、大麻芽和叶）分离的纳米囊泡在葡聚糖硫酸钠（DSS）诱导的小鼠结肠炎中的保护作用。通过纳米颗粒跟踪分析测量，根衍生的纳米囊泡（RNV）、种子衍生的纳米囊泡（SNV）、大麻芽衍生的纳米囊泡（HSNV）和叶衍生的纳米囊泡（LNV）的粒径在 100-200nm 范围内。通过在水中提供 5%DSS 诱导 C57BL/N 小鼠急性结肠炎 7 天。RNV 每天口服给药一次，导致小肠和结肠长度恢复。RNV、SNV 和 HSNV 恢复了 DSS 诱导的小肠和结肠损伤中的紧密（ZO-1、claudin-4、occludin）和黏附连接（E-钙粘蛋白和α-微管蛋白）。此外，RNV 显著降低了 DSS 诱导的小肠和结肠损伤中的 NF-κB 激活和氧化应激蛋白。在暴露于 2%DSS 的 T84 结肠细胞中，RNV、SNV 和 HSNV 处理的紧密连接蛋白表达和上皮细胞通透性升高。有趣的是，RNV、SNV、HSNV 和 LNV 降低了 DSS 诱导的肝损伤中的 ALT 活性和肝再生标记蛋白。这些结果首次表明，大麻衍生的纳米囊泡（HNVs）通过抑制氧化应激标记蛋白，通过肠肝轴对 DSS 诱导的肠道渗漏和肝损伤表现出保护作用。
