State Key Laboratory of Eco-Hydraulics in Northwest Arid Region, Xi'an University of Technology, Xi'an 710048, China.
College of Horticulture, Northwest A&F University, Yangling, Xianyang 712100, China.
Int J Mol Sci. 2022 Sep 2;23(17):10048. doi: 10.3390/ijms231710048.
Saline-alkaline stress suppresses rice growth and threatens crop production. Despite substantial research on rice's tolerance to saline-alkaline stress, fewer studies have examined the impact of magnetic water treatments on saline-alkaline-stressed rice plants. We explored the physiological and molecular mechanisms involved in saline-alkaline stress tolerance enhancement via irrigation with magnetized water using Nipponbare. The growth of Nipponbare plants was inhibited by saline-alkaline stress, but this inhibition was alleviated by irrigating the plants with magnetized water, as evidenced by greater plant height, biomass, chlorophyll content, photosynthetic rates, and root system in plants irrigated with magnetized water compared to those irrigated with non-magnetized water. Plants that were irrigated with magnetized water were able to acquire more total nitrogen. In addition, we proved that rice seedlings irrigated with magnetized water had a greater root NO-nitrogen concentration and root NH-nitrogen concentration than plants irrigated with non-magnetized water. These findings suggest that treatment with magnetized water could increase nitrogen uptake. To test this hypothesis, we analyzed the expression levels of genes involved in nitrogen acquisition. The expression levels of , , , , , , , , , and were higher in plants exposed to magnetized water medium compared to those exposed to non-magnetized water media. We further demonstrated that treatment with magnetized water increases available nitrogen, NO-nitrogen content, and NH-nitrogen content in soil under saline-alkaline stress. Our results revealed that the increased resistance of rice seedlings to saline-alkaline stress may be attributable to a very effective nitrogen acquisition system enhanced by magnetized water.
盐碱胁迫抑制水稻生长并威胁作物产量。尽管有大量关于水稻耐盐碱胁迫的研究，但关于磁化水对盐碱胁迫水稻植株影响的研究较少。我们用日本晴水稻研究了通过磁化水灌溉来提高水稻耐盐碱胁迫的生理和分子机制。日本晴水稻的生长受到盐碱胁迫的抑制，但用磁化水灌溉可以缓解这种抑制，因为用磁化水灌溉的水稻的株高、生物量、叶绿素含量、光合速率和根系都比用非磁化水灌溉的水稻大。用磁化水灌溉的植物能够吸收更多的总氮。此外，我们证明，用磁化水灌溉的水稻幼苗的根硝态氮浓度和根铵态氮浓度都高于用非磁化水灌溉的植物。这些发现表明，磁化水的处理可以增加氮的吸收。为了验证这一假设，我们分析了参与氮吸收的基因的表达水平。与非磁化水相比，磁化水培养基中 、 、 、 、 、 、 、 和 的表达水平更高。我们进一步证明，磁化水的处理增加了盐碱胁迫下土壤中有效氮、硝态氮含量和铵态氮含量。我们的结果表明，水稻幼苗对盐碱胁迫的抗性增加可能归因于磁化水增强的非常有效的氮吸收系统。
