中国本土草本植物白及（兰科：白及属）叶绿体全基因组特征分析
Characterization of the complete chloroplast genome of (Orchidaceae: Bletilla), the herb in China.
作者信息
Feng Hui, Cao Tianyi, Xu Hong, Liu Yihui, Han Ying, Sun Wei, Liu Yi
机构信息
Hangzhou Red Cross Hospital, Hangzhou, PR China.
Zhejiang Chinese Medical University, Hangzhou, PR China.
出版信息
Mitochondrial DNA B Resour. 2019 Oct 12;4(2):3542-3543. doi: 10.1080/23802359.2019.1676177.
Chloroplast (cp) genome sequences have been become a useful tool for phylogenetic and evolutionary studies in the herb. In this study, the complete cp genome of was assembled. The complete cp genome is circular of 157,016 base pairs (bp) in length, which harbours a large single-copy region (85,821 bp), a small single-copy region (17,743 bp), and two inverted-repeat regions (each one of 26,726 bp). The cp genome of contains 139 genes, including 94 protein-coding genes (PCGs), 37 transfer RNA (tRNAs), and 8 ribosome RNA (rRNAs). The overall nucleotide content of the cp genome is A of 31.0%, T of 31.8%, C of 18.9%, and G of 18.3%, with a total AT content of 62.8% and GC content of 37.2%. However, the phylogenetic relationship analysis using maximum-likelihood (ML) method, which the result showed that the position of was situated as in Orchidaceae. This study will be helpful for studies on the cp genetic engineering and medicinal herb value of Orchidaceae species in further.
叶绿体（cp）基因组序列已成为该草本植物系统发育和进化研究的有用工具。在本研究中，组装了[植物名称]的完整cp基因组。完整的cp基因组呈环状，长度为157,016个碱基对（bp），包含一个大单拷贝区域（85,821 bp）、一个小单拷贝区域（17,743 bp）和两个反向重复区域（每个26,726 bp）。[植物名称]的cp基因组包含139个基因，包括94个蛋白质编码基因（PCGs）、37个转运RNA（tRNAs）和8个核糖体RNA（rRNAs）。cp基因组的总体核苷酸含量为A 31.0%、T 31.8%、C 18.9%、G 18.3%，AT总含量为62.8%，GC含量为37.2%。然而，使用最大似然（ML）方法进行的系统发育关系分析结果表明，[植物名称]在兰科中的位置与[对比植物名称]相同。本研究将有助于进一步开展兰科植物cp基因工程和药用价值的研究。
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