Hu Xiaoliang, Lian Yuexiao, He Yucan, Liu Xiangxiao, Tian Zhige, Dai Yi, Liu Mengyuan, Fan Huayan, Shi Yue, Cong Feng
Faculty of Agriculture, Forestry and Food Engineering, Yibin Key Laboratory of Zoological Diversity and Ecological Conservation, Yibin Animal and Plant Inspection and Quarantine Engineering Technology Research Center, Yibin University, Yibin 644000, China.
Guangdong Laboratory Animals Monitoring Institute, Guangdong Provincial Key Laboratory of Laboratory Animals, Guangzhou 510633, China.
Animals (Basel). 2022 Aug 25;12(17):2189. doi: 10.3390/ani12172189.
Since 2010, a variant of porcine epidemic diarrhea virus (PEDV) has re-emerged in several provinces of China, resulting in severe economic losses for the pork industry. Here, we isolated and identified a variant PEDV strain, SC-YB73, in Guangdong Province, China. The pathological observations of jejunum showed atrophy of villi and edema in the lamina propria. The sequence analysis of the viral genome identified a six-nucleotide insertion in the E gene, which has not previously been detected in PEDV strains. Furthermore, 50 nucleotide sites were unique in SC-YB73 compared with 27 other PEDV strains. The phylogenetic analysis based on the complete genome showed that SC-YB73 was clustered in variant subgroup GII-a, which is widely prevalent in the Chinese pig population. The recombination analysis suggested that SC-YB73 originated from the recombination of GDS47, US PEDV prototype-like strains TW/Yunlin550/2018, and COL/Cundinamarca/2014. In the present study, we isolated and genetically characterized a variant PEDV strain, thus providing essential information for the control of PED outbreaks in China.
自2010年以来，一种猪流行性腹泻病毒（PEDV）变体在中国多个省份再度出现，给猪肉行业造成了严重经济损失。在此，我们在中国广东省分离并鉴定出一株PEDV变体毒株SC-YB73。空肠的病理观察显示绒毛萎缩和固有层水肿。病毒基因组序列分析确定E基因中有一个六核苷酸插入，这在之前的PEDV毒株中未曾检测到。此外，与其他27株PEDV毒株相比，SC-YB73有50个核苷酸位点是独特的。基于全基因组的系统发育分析表明，SC-YB73聚类在变体亚组GII-a中，该亚组在中国猪群中广泛流行。重组分析表明，SC-YB73起源于GDS47、美国PEDV原型样毒株TW/Yunlin550/2018和COL/Cundinamarca/2014的重组。在本研究中，我们分离并对一株PEDV变体毒株进行了基因特征分析，从而为中国控制PED疫情提供了重要信息。
