Department of Clinical Laboratory and Food Safety, Faculty of Pharmacy, "Carol Davila" University of Medicine and Pharmacy, 6 Traian Vuia Street, 020945 Bucharest, Romania.
Department of Pharmaceutical Technology and Biopharmacy, Faculty of Pharmacy, "Carol Davila" University of Medicine and Pharmacy, 6 Traian Vuia Street, 020945 Bucharest, Romania.
Int J Environ Res Public Health. 2022 Sep 5;19(17):11114. doi: 10.3390/ijerph191711114.
Cereals whole grains contain vitamins, phytochemicals, antioxidants, resistant starch, and minerals with potential benefits to human health. The consumption of whole grains is correlated with a lowered risk of the most important chronic diseases, including type II diabetes, cardiovascular diseases, and some cancers. This study aimed to characterize and evaluate the content of five cultivars of wheat ( L.) and five cultivars of barley ( L.) obtained by conventional plant breeding using crossing and selection methods. The novelty and the purpose of this research was to quantitatively and qualitatively analyze these ten cultivars from Romania and to show the importance of, and the changes produced by, crossing and selection methods when these are aimed at the physiological or morphological development of the cultivars. Studies based on gluten dosing; spectrophotometry using Bradford, fructan and protein dosing; Kjeldahl protein dosing; GC-MS/MS-protein and amino acid dosing; and identification of protein fractions using polyacrylamide gel electrophoretic method were conducted. This study demonstrates the possibility of developing future cultivars using conventional methods of improvement to modify the content and composition of nutrients to increase their health benefits.
全谷物包含维生素、植物化学物质、抗氧化剂、抗性淀粉和矿物质，对人类健康具有潜在益处。全谷物的消费与降低 II 型糖尿病、心血管疾病和某些癌症等最重要的慢性病风险相关。本研究旨在通过常规植物杂交和选择育种方法，对十种罗马尼亚小麦（ L.）和大麦（ L.）品种进行特征描述和含量评估。本研究的新颖性和目的是定量和定性分析这十种罗马尼亚品种，并展示杂交和选择方法在针对品种的生理或形态发育时所产生的重要性和变化。研究基于谷朊粉剂量测定、Bradford 分光光度法、果糖和蛋白质剂量测定、凯氏定氮蛋白质剂量测定、GC-MS/MS-蛋白质和氨基酸剂量测定以及使用聚丙烯酰胺凝胶电泳方法鉴定蛋白质馏分。本研究表明，使用常规改良方法开发未来品种以改变营养物质的含量和组成以增加其健康益处是可行的。
