Gupta Nakul, Singh Prabhakar Mohan, Sagar Vidya, Pandya Alok, Chinnappa Manimurugan, Kumar Rajesh, Bahadur Anant
ICAR-Indian Institute of Vegetable Research, PB-01, Po-Jakhini (Sahanshahpur), Varanasi 221305, Uttar Pradesh, India.
Department of Engineering & Physical Sciences, Institute of Advanced Research, Koba Institutional Area, Gandhinagar 382426, Gujarat, India.
Plants (Basel). 2022 Aug 28;11(17):2227. doi: 10.3390/plants11172227.
The increased lead (Pb) content in the environment has an impact on all living beings, including plant growth and quality. The present study aims to investigate the protective roles of zinc (Zn)- and iron (Fe)- nanoparticles (NPs) in alleviating stress symptoms caused by lead (Pb) exposure in Basella alba seedlings. For this purpose, 15 different treatment combinations of seed priming with two NPs at 0 and 200 mg L−1, and five Pb levels (0, 4, 8, 15, 20 mM) were chosen. Pb stress (20 mM) was found to reduce seed germination by 72.8% and seedling growth, particularly root length, by 92% when compared to the control. Under different Pb concentrations, seed priming with ZnNPs (200 mg L−1) and FeNPs (200 mg L−1) increased seed germination by 34.7% and 54.9%, respectively, and root length by 152.9% and 252.9%, respectively. In 20 mM Pb stress, NPs primed seedling showed decrease in Pb content by 33.7% with ZnNPs and 32.6% with FeNPs. Increased Pb stress resulted in increased reactive oxygen species (ROS) generation (H2O2) and lipid peroxidation (MDA) compared to non-Pb stressed seedlings. However, increased antioxidants in the NPs treatments such as SOD, CAT, POD and proline content, scavenged these ROS. Considering all the parameters under study, priming alleviated Pb stress in the following order: FeNPs > ZnNPs > hydropriming > control. To summarise, seed priming with Zn- and Fe-NPs has the potential to alleviate Pb toxicity via reduced Pb uptake, ROS generation and lipid peroxidation as well as increased proline content and activation of antioxidant enzymatic system.
环境中铅（Pb）含量的增加对所有生物都有影响，包括植物的生长和品质。本研究旨在探究锌（Zn）和铁（Fe）纳米颗粒（NPs）在减轻落葵幼苗铅（Pb）暴露引起的胁迫症状方面的保护作用。为此，选择了两种纳米颗粒在0和200 mg L−1浓度下进行种子引发处理，以及五个铅水平（0、4、8、15、20 mM），共15种不同的处理组合。与对照相比，发现铅胁迫（20 mM）使种子发芽率降低了72.8%，幼苗生长尤其是根长降低了92%。在不同铅浓度下，用ZnNPs（200 mg L−1）和FeNPs（200 mg L−1）进行种子引发处理分别使种子发芽率提高了34.7%和54.9%，根长分别提高了152.9%和252.9%。在20 mM铅胁迫下，纳米颗粒引发处理的幼苗中，ZnNPs使铅含量降低了33.7%，FeNPs使铅含量降低了32.6%。与未受铅胁迫的幼苗相比，增加的铅胁迫导致活性氧（ROS）生成（H2O2）和脂质过氧化（MDA）增加。然而，纳米颗粒处理中抗氧化剂如超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、过氧化物酶（POD）和脯氨酸含量的增加清除了这些ROS。综合考虑所有研究参数，引发处理减轻铅胁迫的顺序为：FeNPs > ZnNPs > 水引发 > 对照。总之，用Zn和Fe纳米颗粒进行种子引发处理有可能通过减少铅吸收、ROS生成和脂质过氧化，以及增加脯氨酸含量和激活抗氧化酶系统来减轻铅毒性。
