College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou, 350002, China and Age-Related Inflammation and Degeneration Research Unit, Department of Clinical Chemistry, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok, 10330, Thailand and Institute of Pharmacy and Molecular Biotechnology, Im Neuenheimer Feld 329, Heidelberg University, Heidelberg, 69120, Germany.
Age-Related Inflammation and Degeneration Research Unit, Department of Clinical Chemistry, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok, 10330, Thailand and Institute of Pharmacy and Molecular Biotechnology, Im Neuenheimer Feld 329, Heidelberg University, Heidelberg, 69120, Germany.
Food Funct. 2020 Sep 23;11(9):8179-8192. doi: 10.1039/d0fo01072c.
Oolong tea, a traditional Chinese tea, is especially popular in south China and has a variety of health benefits. However, studies about its neuroprotective and neuroregenerative properties are still limited. This study explored the neuroprotective and neurite outgrowth-promoting properties of oolong tea in cultured neuronal cells (Neuro-2a and HT22) and Caenorhabditis elegans models. Ultra performance liquid chromatography was applied to identify the main natural bioactive compounds in oolong tea. Using Neuro-2a and HT22 cells, we found that oolong tea extracts had a protective effect against glutamate-induced cell death. The extracts reduced intracellular reactive oxygen species accumulation and induced gene expression of cellular antioxidant enzymes such as GPx, GSTs and SODs. These extracts also increased the average neurite length, and GAP-43 and Ten-4 mRNA expression in Neuro-2a cells. Moreover, they had protective effects against Aβ-induced paralysis, chemotaxis deficiency and α-synuclein aggregation in C. elegans. This is the first study showing the neuroregenerative and neuroprotective potential of the oolong tea extracts against glutamate/Aβ/α-synuclein-induced toxicity in vitro and in vivo. Our study may support oolong tea extracts as potential candidates for the prevention of neurodegenerative diseases.
乌龙茶，一种中国传统茶，尤其在中国南方深受欢迎，具有多种健康益处。然而，关于其神经保护和神经再生特性的研究仍然有限。本研究探讨了乌龙茶在培养神经元细胞（Neuro-2a 和 HT22）和秀丽隐杆线虫模型中的神经保护和促进神经突生长的特性。超高效液相色谱法被用于鉴定乌龙茶中的主要天然生物活性化合物。使用 Neuro-2a 和 HT22 细胞，我们发现乌龙茶提取物对谷氨酸诱导的细胞死亡具有保护作用。提取物减少了细胞内活性氧的积累，并诱导了细胞抗氧化酶如 GPx、GSTs 和 SODs 的基因表达。这些提取物还增加了 Neuro-2a 细胞中平均神经突长度和 GAP-43 和 Ten-4 mRNA 的表达。此外，它们对秀丽隐杆线虫中 Aβ诱导的麻痹、趋化性缺陷和α-突触核蛋白聚集具有保护作用。这是首次研究表明，乌龙茶提取物具有体外和体内对抗谷氨酸/Aβ/α-突触核蛋白诱导的毒性的神经再生和神经保护潜力。我们的研究可能支持乌龙茶提取物作为预防神经退行性疾病的潜在候选物。
