Unidad de Biotecnología, Centro de Investigación Científica de Yucatán, Chuburná de Hidalgo, Mérida 97205, Mexico.
Departamento de Virología, Centro de Investigaciones Regionales, Universidad Autónoma de Yucatán, Paseo de Las Fuentes, Mérida 97225, Mexico.
Molecules. 2022 Aug 26;27(17):5494. doi: 10.3390/molecules27175494.
(Greenm.) Gagnon & GP Lewis is a legume tree native to and widely distributed in southeast Mexico, where its branches are used in traditional medicine. An in vitro evaluation of the antiviral activity of extracts and fractions from the leaves, stem bark and roots against two strains of the AH1N1 influenza virus was performed, leading to the identification of bioactive compounds in this medicinal plant. In a cytopathic effect reduction assay, the fractions from the leaves and stem bark were the active elements at the co-treatment level. These were further fractionated based on their hemagglutination inhibition activity. The analysis of spectroscopy data identified a combination of phytosterols (β-sitosterol, stigmasterol and campesterol) in the stem bark active fraction as the main anti-hemagglutinin binding components, while 5-hydroxy-2(2-hydroxy-3,4,5-trimethoxyphenyl)-7-metoxi-4H(chromen-4-ona), which was isolated from the leaf extracts, showed a weak inhibition of viral hemagglutinin. Time of addition experiments demonstrated that the mixture of sterols had a direct effect on viral particle infectivity at the co-treatment level (IC50 = 3.125 µg/mL). This effect was also observed in the virus plaque formation inhibition assay, where the mixture showed 90% inhibition in the first 20 min of co-treatment at the same concentration. Additionally, it was found using qRT-PCR that the NP copy number was reduced by 92.85% after 60 min of co-treatment. These results are the first report of components with anti-hemagglutinin binding activity in the genus sp.
(Greenm.) Gagnon & GP Lewis 是一种原产于墨西哥东南部并广泛分布于该地区的豆科树木，其树枝在传统医学中被使用。对来自叶片、茎皮和根的提取物和馏分对两种 AH1N1 流感病毒株的抗病毒活性进行了体外评估，从而鉴定了这种药用植物中的生物活性化合物。在细胞病变效应减少测定中，来自叶片和茎皮的馏分是共同处理水平下的活性成分。根据其血凝抑制活性，进一步对这些馏分进行了分段。光谱数据分析表明，茎皮活性馏分中的一组植物甾醇（β-谷甾醇、豆甾醇和菜油甾醇）是主要的抗血凝素结合成分，而从叶片提取物中分离出的 5-羟基-2(2-羟基-3,4,5-三甲氧基苯基)-7-甲氧基-4H(色满-4-酮)表现出对病毒血凝素的弱抑制作用。添加时间实验表明，甾醇混合物在共同处理水平上对病毒粒子感染力具有直接作用（IC50 = 3.125 µg/mL）。在病毒斑形成抑制测定中也观察到了这种效应，在相同浓度下，混合物在共同处理的前 20 分钟内表现出 90%的抑制作用。此外，qRT-PCR 发现，在共同处理 60 分钟后，NP 拷贝数减少了 92.85%。这些结果是首次报道属 sp. 中具有抗血凝素结合活性的成分。
