高良姜提取物抗流感病毒感染和血凝作用的体外抑制活性。
In vitro inhibitory activity of Alpinia katsumadai extracts against influenza virus infection and hemagglutination.
机构信息
Eco-Friendly Biomaterial Research Center and AI Control Material Research Center, Korea Research Institute of Bioscience and Biotechnology, Jeongeup 580-185, Republic of Korea.
出版信息
Virol J. 2010 Nov 10;7:307. doi: 10.1186/1743-422X-7-307.
BACKGROUND
Alpinia katsumadai (AK) extracts and fractions were tested for in vitro antiviral activities against influenza virus type A, specially human A/PR/8/34 (H1N1) and avian A/Chicken/Korea/MS96/96 (H9N2), by means of time-of-addition experiments; pre-treatment, simultaneous treatment, and post treatment.
RESULTS
In pre-treatment assay, the AK extracts and AK fractions did not show significant antiviral activity. During the simultaneous treatment assay, one AK extract and five AK fractions designated as AK-1 to AK-3, AK-5, AK-10, and AK-11 showed complete inhibition of virus infectivity against A/PR/8/34 (H1N1) and A/Chicken/Korea/MS96/96 (H9N2). The 50% effective inhibitory concentrations (EC₅₀) of these one AK extracts and five AK fractions with exception of the AK-9 were from 0.8 ± 1.4 to 16.4 ± 4.5 μg/mL against A/PR/8/34 (H1N1). The two AK extracts and three AK fractions had EC₅₀ values ranging from <0.39 ± 0.4 to 2.3 ± 3.6 μg/mL against A/Chicken/Korea/MS96/96 (H9N2). By the hemagglutination inhibition (HI) assay, the two AK extracts and five AK fractions completely inhibited viral adsorption onto chicken RBCs at less than 100 μg/mL against both A/PR/8/34 (H1N1) and A/Chicken/Korea/MS96/96 (H9N2). Interestingly, only AK-3 was found with inhibition for both viral attachment and viral replication after showing extended antiviral activity during the post treatment assay and quantitative real-time PCR.
CONCLUSIONS
These results suggest that AK extracts and fractions had strong anti-influenza virus activity that can inhibit viral attachment and/or viral replication, and may be used as viral prophylaxis.
背景
采用时效关系实验，对高良姜（AK）提取物及其馏分进行了抗甲型流感病毒（特别是人 A/PR/8/34（H1N1）和禽 A/鸡/韩国/MS96/96（H9N2））的体外抗病毒活性测试，进行了预处理、同时处理和后处理。
结果
在预处理试验中，AK 提取物和 AK 馏分没有显示出显著的抗病毒活性。在同时处理试验中，一种 AK 提取物和五种 AK 馏分，分别命名为 AK-1 至 AK-3、AK-5、AK-10 和 AK-11，对 A/PR/8/34（H1N1）和 A/鸡/韩国/MS96/96（H9N2）显示出完全抑制病毒感染力的作用。除 AK-9 外，这些 AK 提取物和 AK 馏分中的一种对 A/PR/8/34（H1N1）的 50%有效抑制浓度（EC₅₀）为 0.8 ± 1.4 至 16.4 ± 4.5μg/ml，对 A/鸡/韩国/MS96/96（H9N2）的 EC₅₀ 值为 0.39 ± 0.4 至 2.3 ± 3.6μg/ml。两种 AK 提取物和三种 AK 馏分对 A/鸡/韩国/MS96/96（H9N2）的 EC₅₀ 值均小于 0.39 ± 0.4μg/ml。通过血凝抑制（HI）试验，两种 AK 提取物和五种 AK 馏分在小于 100μg/ml 时完全抑制了病毒吸附到鸡 RBC 上，对 A/PR/8/34（H1N1）和 A/鸡/韩国/MS96/96（H9N2）均有抑制作用。有趣的是，只有 AK-3 在后续处理试验和实时定量 PCR 中显示出延长的抗病毒活性后，才发现对病毒附着和病毒复制均有抑制作用。
结论
这些结果表明，AK 提取物及其馏分具有较强的抗流感病毒活性，可抑制病毒附着和/或病毒复制，可作为病毒预防用药。
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