Hwa Mei Hospital, University of Chinese Academy of Sciences, Ningbo 315010, China.
Department of Chemistry, Zhejiang University, Hangzhou 310027, China.
Molecules. 2022 Aug 27;27(17):5509. doi: 10.3390/molecules27175509.
In this work, magnetic tetraethylenepentamine (TEPA)-modified carboxyl-carbon nanotubes were synthesized, characterized, and used as adsorbents to conduct magnetic solid-phase extraction (MSPE) for the preconcentration of seven local anesthetic drugs (procaine, lidocaine, mepivacaine, oxybuprocaine, bupivacaine, tetracaine, and cinchocaine) from human plasma. The separation and determination of analytes were performed on high-performance liquid chromatography with UV detection. Several factors affected the extraction efficiency, such as the amount of adsorbents used, extraction time, sample pH, and optimization of elution conditions. Under optimal conditions, satisfactory linear relationships were obtained in the range of 0.02-5.00 mg/L, with the limits of detection (LOD) ranging from 0.003 mg/L to 0.008 mg/L. The recoveries of analytes for spiked human plasma were in the range of 82.0-108%. Moreover, the precision with intra-day and inter-day RSD values were obtained in the range of 1.5-7.7% and 1.5-8.3%. The results indicated that this method could determine the concentration of seven local anesthetic drugs in human plasma with high precision and repeatability and provide support for the clinical monitoring of the concentration of local anesthetic drugs in human plasma.
在这项工作中，合成了磁性四乙烯五胺（TEPA）修饰的羧基-碳纳米管，并将其用作吸附剂，通过磁固相萃取（MSPE）从人血浆中预浓缩七种局部麻醉药（普鲁卡因、利多卡因、甲哌卡因、氧布卡因、布比卡因、四卡因和辛可卡因）。采用高效液相色谱法，紫外检测法对分析物进行分离和测定。影响萃取效率的因素有很多，如吸附剂的用量、萃取时间、样品 pH 值以及洗脱条件的优化等。在最佳条件下，在 0.02-5.00 mg/L 范围内得到了令人满意的线性关系，检出限（LOD）范围为 0.003-0.008 mg/L。对于添加到人血浆中的分析物的回收率在 82.0-108%之间。此外，日内和日间 RSD 值的精密度在 1.5-7.7%和 1.5-8.3%的范围内。结果表明，该方法可用于测定人血浆中七种局部麻醉药的浓度，具有较高的精密度和重复性，可为临床监测人血浆中局部麻醉药浓度提供支持。
