College of Food Science and Technology, Hunan Agricultural University, Changsha 410128, China.
Department of Food Science and Technology, Hunan Food and Drug Vocational College, Changsha 410128, China.
Molecules. 2022 Aug 29;27(17):5560. doi: 10.3390/molecules27175560.
is an important economic forestry product in China, whose seeds are often consumed as edible nuts, or used as raw materials for oil processing. To date, as an important by-product of , comprehensive studies regarding the seed coat phenolic composition are lacking, which greatly limits its in-depth use. Therefore, in the present study, the seed coat was extracted by acid aqueous ethanol (TE), and NMR and UHPLC-MS were used to identify the major phenolics. Together with the already known phenolics including protocatechuic acid, catechin, epigallocatechin gallate, and epicatechin gallate, the unreported new compound 2-hydroxy-2-(4-hydroxyphenylethyl) malonic acid was discovered. The results of the antioxidant properties showed that both TE and 2-hydroxy-2-(4-hydroxyphenylethyl) malonic acid exhibited strong ABTS, DPPH, and hydroxyl radical-scavenging activity, and significantly improved the O/W emulsion's oxidation stability. These results indicate that the TE and 2-hydroxy-2-(4-hydroxyphenylethyl) malonic acid could possibly be used in the future to manufacture functional foods or bioactive ingredients. Moreover, further studies are also needed to evaluate the biological activity of TE and 2-hydroxy-2-(4-hydroxyphenylethyl) malonic acid to increase the added value of by-products.
是中国重要的经济林产品，其种子常被作为食用坚果，或作为油料加工的原料。迄今为止，作为 的重要副产物，关于 种皮酚类成分的综合研究还很缺乏，这极大地限制了其深入应用。因此，本研究采用酸性水乙醇（TE）提取 种皮，并采用 NMR 和 UHPLC-MS 鉴定主要酚类物质。除了已报道的包括原儿茶酸、儿茶素、没食子酸表儿茶素酯和表儿茶素没食子酸酯在内的酚类物质外，还发现了一种未报道的新化合物 2-羟基-2-(4-羟基苯乙基)丙二酸。抗氧化性能结果表明，TE 和 2-羟基-2-(4-羟基苯乙基)丙二酸均具有较强的 ABTS、DPPH 和羟基自由基清除活性，显著提高了 O/W 乳液的氧化稳定性。这些结果表明，TE 和 2-羟基-2-(4-羟基苯乙基)丙二酸未来可能被用于制造功能性食品或生物活性成分。此外，还需要进一步研究 TE 和 2-羟基-2-(4-羟基苯乙基)丙二酸的生物活性，以提高 的附加值。
