State Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Lin'an, Zhejiang Province 311300, China.
Zhejiang Provincial Key Laboratory of Resources Protection and Innovation of Traditional Chinese Medicine, Zhejiang A&F University, Hangzhou 311300, China.
Food Chem. 2023 Mar 30;405(Pt A):134843. doi: 10.1016/j.foodchem.2022.134843. Epub 2022 Nov 3.
Torreya grandis nut is a chief functional food in China consumed for centuries. Besides its rich protein composition, increasing studies are now focusing on T. grandis functional proteins that have not yet identified. In this study, liquid chromatography coupled with mass spectrometry detection of smaller and major proteins, revealed that the major peptide was 36935.00 Da. Proteome sequencing annotated 142 proteins in total. Bioactive proteins such as defensin 4 was annotated and its anti-microbial function was verified. Finally, functional oligopeptides were predicted by searching sequences of digested peptides in databases. Ten group of oligopeptides were suggested to exhibit antioxidant, Angiotensin-converting enzyme inhibition, anti-inflammatory. The predicted antioxidant activity was experimentally validated. It is interesting that a peptide GYCVSDNN digested from defensin 4 showed antioxidant activity. This study reports novel functional peptides from T. grandis nuts that have not been isolated and/or included as functional ingredients in nutraceuticals and in food industry.
香榧子是中国的一种主要功能性食品，已经食用了几个世纪。除了其丰富的蛋白质组成外，现在越来越多的研究集中在尚未确定的香榧功能性蛋白质上。在这项研究中，通过液相色谱与质谱联用检测小分子和主要蛋白质，发现主要肽段为 36935.00 Da。蛋白质组学测序共注释了 142 种蛋白质。防御素 4 等生物活性蛋白被注释，并验证了其抗菌功能。最后，通过在数据库中搜索消化肽的序列来预测功能寡肽。共提出了十组具有抗氧化、血管紧张素转换酶抑制、抗炎作用的寡肽。预测的抗氧化活性通过实验得到了验证。有趣的是，从防御素 4 中消化得到的肽 GYCVSDNN 具有抗氧化活性。本研究报道了香榧子中的新型功能性肽，这些肽尚未被分离出来，并作为功能性成分添加到营养保健品和食品工业中。
