Shi Haiming, Wang Huandi, Wang Mengyue, Li Xiaobo
School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, PR China.
Nat Prod Commun. 2009 Nov;4(11):1565-70.
Torreya grandis cv. Merrillii is an endemic tree species in China, seeds of which are used as a popular snack, possessing beneficial effects on preventing angiosclerosis and coronary heart diseases. In this study, antioxidant activity and chemical constituents of T. grandis cv. Merrillii seed (TGMS) were investigated. The antioxidant activity of different fractions and the ethanol extract was evaluated using 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging capacity, hydroxyl radical scavenging activity and lipid peroxidation assays. The oil, CH2Cl2 and n-BuOH fractions, and ethanol extract of TGMS all showed antioxidant activities in these models, especially the DPPH one. By GC-MS analysis, twenty-seven constituents were identified from the oil fraction of TGMS. The total content of phenolic compounds in the CH2Cl2 and n-BuOH fractions and ethanol extract was also determined by the Folin-Ciocalteau method as 17.6, 21.6 and 12.9 microg/mg, respectively. In addition, analysis of the CH2Cl2 fraction yielded four phenolic compounds: 4-hydroxybenzaldehyde, 4-methoxy pyrocatechol, coniferyl aldehyde, 4-hydroxy cinnamaldehyde, and two steroids, beta-sitosterol and daucosterol. These results provide scientific support for the empirical use of TGMS as a medicinal food for cardiovascular diseases.
香榧是中国特有的树种，其种子作为一种受欢迎的小吃，对预防动脉硬化和冠心病具有有益作用。在本研究中，对香榧种子（TGMS）的抗氧化活性和化学成分进行了研究。使用1,1-二苯基-2-苦基肼（DPPH）自由基清除能力、羟基自由基清除活性和脂质过氧化测定法评估了不同馏分和乙醇提取物的抗氧化活性。TGMS的油、二氯甲烷和正丁醇馏分以及乙醇提取物在这些模型中均表现出抗氧化活性，尤其是DPPH模型。通过气相色谱-质谱联用（GC-MS）分析，从TGMS的油馏分中鉴定出27种成分。还用福林-西奥尔特法测定了二氯甲烷和正丁醇馏分以及乙醇提取物中酚类化合物的总含量，分别为17.6、21.6和12.9微克/毫克。此外，对二氯甲烷馏分的分析产生了四种酚类化合物：4-羟基苯甲醛、4-甲氧基邻苯二酚、松柏醛、4-羟基肉桂醛，以及两种甾体化合物，β-谷甾醇和胡萝卜苷。这些结果为香榧作为心血管疾病药用食品的经验性使用提供了科学支持。
