Institute of Ecology and Biological Resources, Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, Cau Giay, Ha Noi 10072, Vietnam.
Graduate University of Science and Technology, VAST, 18 Hoang Quoc Viet, Ha Noi 10072, Vietnam.
Molecules. 2022 Aug 31;27(17):5599. doi: 10.3390/molecules27175599.
This study was performed to investigate the effects of different supplemental light spectra and doses (duration and illuminance) on the essential oil of basil (Ocimum basilicum L.) cultivated in the net-house in Vietnam during four months. Ten samples of basil aerial parts were hydrodistilled to obtain essential oils which had the average yields from 0.88 to 1.30% (v/w, dry). The oils analyzed using GC-FID and GC-MS showed that the main component was methyl chavicol (87.4−90.6%) with the highest values found in the oils of basil under lighting conditions of 6 h/day and 150−200 µmol·m−2·s−1. Additional lighting conditions caused the significant differences (p < 0.001) in basil biomass and oil production with the highest values found in the oils of basil under two conditions of (1) 71% Red: 20% Blue: 9.0% UVA in at 120 μmol·m−2·s−1 in 6 h/day and (2) 43.5% Red: 43.5% Blue: 8.0% Green: 5.0% Far-Red at 100 μmol·m−2·s−1 in 6 h/day. The oils of basil in some formulas showed weak inhibitory effects on only the Bacillus subtilis strain. Different light spectra affect the biomass and essential oil production of basil, as well as the concentrations of the major components in the oil.
本研究旨在调查不同补充光光谱和剂量（持续时间和光照度）对越南网室内种植的罗勒（Ocimum basilicum L.）精油的影响。从罗勒地上部分中提取了 10 个样品，通过水蒸馏获得精油，平均产率为 0.88-1.30%（v/w，干重）。用 GC-FID 和 GC-MS 分析的油表明，主要成分是甲基丁香酚（87.4-90.6%），在每天光照 6 小时、光照度为 150-200 μmol·m−2·s−1 的条件下，罗勒油中的含量最高。额外的光照条件导致罗勒生物量和产油量的显著差异（p < 0.001），在两种条件下，罗勒油中的含量最高：（1）120 μmol·m−2·s−1 光照 6 小时，红光：蓝光：UVA 为 71%：20%：9.0%；（2）红光：蓝光：绿光：远红光为 43.5%：43.5%：8.0%：5.0%，光照度为 100 μmol·m−2·s−1。一些配方的罗勒油对枯草芽孢杆菌菌株仅显示出较弱的抑制作用。不同的光光谱影响罗勒的生物量和精油产量，以及油中主要成分的浓度。
