Department of Pharmaceutical Technology, Faculty of Pharmacy, Silpakorn University, Nakhon Pathom 73000, Thailand.
Pharmaceutical Biopolymer Group (PBiG), Faculty of Pharmacy, Silpakorn University, Nakhon Pathom 73000, Thailand.
Molecules. 2022 Jun 29;27(13):4162. doi: 10.3390/molecules27134162.
The major bioactive components of (KP) rhizomes, 3,5,7,3',4'-pentamethoxyflavone (PMF), 5,7-dimethoxyflavone (DMF), and 5,7,4'-trimethoxyflavone (TMF), were chosen as the quantitative and qualitative markers for this plant material. In order to extract bioactive components (total methoxyflavones) from KP rhizomes, ultrasound-assisted extraction (UAE) was proposed as part of this study. Plackett-Burman design (PBD) and Box-Behnken design (BBD) were utilized to optimize the effects of UAE on extraction yields and total methoxyflavone contents in KP rhizomes. First, PBD was utilized to determine the effect of five independent variables on total yields and total methoxyflavone contents. The results indicated that the concentration of the extracting solvent (ethanol), the extraction time, and the ratio of solvent to solid were significant independent terms. Subsequently, BBD with three-level factorial experiments was used to optimize the crucial variables. It was discovered that the concentration of ethanol was the most influential variable on yields and total methoxyflavone contents. Optimum conditions for extraction yield were ethanol concentration (54.24% /), extraction time (25.25 min), and solvent-to-solid ratio (49.63 mL/g), while optimum conditions for total methoxyflavone content were ethanol concentration (95.00% /), extraction time (15.99 min), and solvent-to-solid ratio (50.00 mL/g). The relationship between the experimental and theoretical values was perfect, which proved that the regression models used were correct and that PBD and BBD were used to optimize the conditions in the UAE to obtain the highest yield and total methoxyflavone content in the KP rhizomes.
（KP）根茎的主要生物活性成分，3,5,7,3',4'-五甲氧基黄酮（PMF）、5,7-二甲氧基黄酮（DMF）和 5,7,4'-三甲氧基黄酮（TMF），被选为该植物材料的定量和定性标志物。为了从 KP 根茎中提取生物活性成分（总甲氧基黄酮），本研究提出了超声辅助提取（UAE）。Plackett-Burman 设计（PBD）和 Box-Behnken 设计（BBD）被用于优化 UAE 对 KP 根茎提取产率和总甲氧基黄酮含量的影响。首先，利用 PBD 确定了五个独立变量对总产率和总甲氧基黄酮含量的影响。结果表明，提取溶剂（乙醇）的浓度、提取时间和溶剂与固体的比例是显著的独立项。随后，采用三水平析因实验的 BBD 优化了关键变量。发现乙醇浓度是对产率和总甲氧基黄酮含量影响最大的变量。提取产率的最佳条件为乙醇浓度（54.24% /）、提取时间（25.25 分钟）和溶剂与固体的比例（49.63 毫升/克），而总甲氧基黄酮含量的最佳条件为乙醇浓度（95.00% /）、提取时间（15.99 分钟）和溶剂与固体的比例（50.00 毫升/克）。实验值与理论值之间的关系非常完美，这证明了所使用的回归模型是正确的，并且 PBD 和 BBD 被用于优化 UAE 的条件，以获得 KP 根茎中最高的产率和总甲氧基黄酮含量。
