在冷冻人全血系统中比较高等真菌（担子菌）变应原孢子诱导白细胞介素-1β的效力。
Comparison of the interleukin-1β-inducing potency of allergenic spores from higher fungi (basidiomycetes) in a cryopreserved human whole blood system.
机构信息
Department of Environmental Health Sciences, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, Md., USA.
出版信息
Int Arch Allergy Immunol. 2014;163(2):154-62. doi: 10.1159/000357036. Epub 2013 Dec 17.
BACKGROUND
Spores from basidiomycete fungi (basidiospores) are highly prevalent in the atmosphere of urban and rural settings. Studies have confirmed their potential to affect human health as allergens. Less is known about their potential to serve as stimuli of the innate immune system and induce proinflammatory reactions.
METHODS
In this study, we evaluated the proinflammatory potential of spores from 11 allergenic basidiomycete species (gilled: Pleurotus ostreatus,Oudemansiella radicata,Armillaria tabescens,Coprinus micaceus,Pluteus cervinus, and Chlorophyllum molybdites, and nongilled: Pisolithus arhizus,Merulius tremellosus,Calvatia cyathiformis,Lycoperdon pyriforme, andBoletus bicolor) based on their potency to induce the release of the proinflammatory cytokine interleukin (IL)-1β in a cryopreserved human whole blood system. In addition, the roles of morphological features of the spores (surface area, shape, and pigmentation) were examined for their role in the IL-1β-including potency of spores. Peripheral blood from healthy volunteers was collected, pooled, and cryopreserved. After stimulating the cryopreserved pooled blood with 10(6) to 10(3) basidiospores/ml, the concentration of IL-1β in culture supernatants was determined with ELISA.
RESULTS
Basidiospores manifested concentration-dependent IL-1β-inducing potency, which was more marked among basidiospores from gilled basidiomycetes. At higher concentrations of basidiospores, the IL-1β-inducing potency could be differentiated in the cryopreserved human whole blood system. Morphological features did not correlate with the IL-1β-inducing potency of the basidiospores, suggesting that nonmorphological properties modulate the IL-1β-inducing potency.
CONCLUSION
Our data provide evidence of the proinflammatory potential of basidiospores, and the utility of cryopreserved human whole blood as a human-based in vitro system to study the immune reactivity of allergenic basidiospores.
背景
担子菌真菌（担子孢子）的孢子在城市和农村环境的大气中高度普遍存在。研究证实它们作为过敏原会影响人类健康。关于它们作为先天免疫系统刺激物并诱导促炎反应的潜力知之甚少。
方法
在这项研究中，我们根据 11 种致敏担子菌物种（有褶：糙皮侧耳、红栓菌、阿廷菌、小皮伞、绒皮侧耳和藻状菌，和无褶：巨大口蘑、美味牛肝菌、杯伞、漏斗状马勃、双色牛肝菌）孢子诱导冷冻保存人全血系统中促炎细胞因子白细胞介素（IL）-1β释放的能力，评估了这些孢子的促炎潜力。此外，还检查了孢子的形态特征（表面积、形状和色素沉着）在孢子的 IL-1β包括能力中的作用。从健康志愿者采集、汇集和冷冻保存外周血。用 10(6)至 10(3)个孢子/ml 刺激冷冻保存的汇集血液后，用 ELISA 测定培养上清液中 IL-1β的浓度。
结果
担子孢子表现出浓度依赖性的 IL-1β诱导能力，其中有褶担子菌的担子孢子更为明显。在较高浓度的担子孢子下，在冷冻保存的人全血系统中可以区分出 IL-1β诱导能力。形态特征与担子孢子的 IL-1β诱导能力无关，表明非形态特性调节 IL-1β诱导能力。
结论
我们的数据提供了担子孢子促炎潜力的证据，并提供了冷冻保存人全血作为研究过敏原担子孢子免疫反应性的基于人类的体外系统的实用性。
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