van Heyningen V, Abbott S R, Daniel S G, Ardisson L J, Ridgway E C
Clin Chem. 1987 Aug;33(8):1387-90.
Using improved selection techniques, we isolated four monoclonal antibodies with high affinity for human thyrotropin (Ka = 1.6 X 10(8) to 2.6 X 10(10) L/mol). We used two of these in an immunoradiometric assay (IRMA) that also incorporates a novel phase-separation technology (Sucrosep TSH IRMA, Boots-Celltech). This assay's very low detection limit for TSH (0.03-0.08 milli-int. unit/L) and wide working range (0-250 milli-int. unit/L) allow the differential diagnosis of hypothyroid, euthyroid, and hyperthyroid patients. We compare the utility of this IRMA with that of a RIA for patients with various thyroid disorders. As determined by IRMA, a normal concentration of TSH in serum excludes hyperthyroidism or hypothyroidism, whereas an undetectable serum TSH concentration (less than 0.08 milli-int. unit/L) accurately predicts an abnormality in thyroid gland function.
运用改良的筛选技术，我们分离出了四种对人促甲状腺激素具有高亲和力的单克隆抗体（亲和常数Ka = 1.6×10⁸至2.6×10¹⁰L/mol）。我们将其中两种用于一种免疫放射分析（IRMA）中，该分析还采用了一种新型的相分离技术（蔗糖相促甲状腺激素免疫放射分析，布茨-赛尔泰克公司）。此分析对促甲状腺激素极低的检测限（0.03 - 0.08毫国际单位/升）和宽泛的工作范围（0 - 250毫国际单位/升）使得能够对甲状腺功能减退、甲状腺功能正常和甲状腺功能亢进患者进行鉴别诊断。我们比较了这种免疫放射分析与放射免疫分析（RIA）对患有各种甲状腺疾病患者的效用。通过免疫放射分析测定，血清中促甲状腺激素浓度正常可排除甲状腺功能亢进或减退，而血清促甲状腺激素浓度检测不到（低于0.08毫国际单位/升）则准确预示甲状腺功能异常。
