John R, Jones M K
Clin Chem. 1984 Aug;30(8):1396-8.
In this two-site immunoradiometric assay for thyrotropin, developed for use in the "Kemtek 3000" automated radioimmunoassay system, commercially available monoclonal antibody to thyrotropin is labeled with 125I, and the solid-phase antibody is an IgG fraction of sheep antiserum to thyrotropin, covalently coupled to reprecipitated aminocellulose. There are two incubations, totalling 3 h, the sensitivity is 0.03 milli-int. unit/L. The mean thyrotropin value for 82 healthy euthyroid subjects was 1.7 milli-int. units/L (range 0.4-3.6). For 19 overtly clinically and biochemically hyperthyroid subjects the values ranged from undetectable to 0.2 milli-int. unit/L. In this assay, euthyroid and hyperthyroid subjects can be distinguished with assay of a single basal sample. The assay appears suitable for routine use as a first-line test of thyroid function.
在这种为“Kemtek 3000”自动放射免疫分析系统开发的促甲状腺激素两点免疫放射分析中，市售的促甲状腺激素单克隆抗体用¹²⁵I标记，固相抗体是羊抗促甲状腺激素血清的IgG组分，与再沉淀的氨基纤维素共价偶联。有两次孵育，共3小时，灵敏度为0.03毫国际单位/升。82名健康甲状腺功能正常受试者的促甲状腺激素平均值为1.7毫国际单位/升（范围0.4 - 3.6）。19名明显临床和生化甲亢受试者的值范围从检测不到到0.2毫国际单位/升。在该分析中，通过检测单个基础样本即可区分甲状腺功能正常和甲亢受试者。该分析似乎适合作为甲状腺功能一线检测的常规使用。
