Gao Xiong, Yao Yanting, Chen Xujie, Lin Xiaorong, Yang Xiaobing, Ho Chi-Tang, Li Bin, Chen Zhongzheng
State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Safety and Health, Key Laboratory of Agricultural Microbiomics and Precision Application, Ministry of Agriculture and Rural Affairs, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou, China.
College of Food Science, South China Agricultural University, Guangzhou, China.
Front Nutr. 2022 Aug 23;9:987807. doi: 10.3389/fnut.2022.987807. eCollection 2022.
Selenium nanoparticles (SeNPs) have gained extensive attention for their excellent biological activity and low toxicity. However, SeNPs are extremely liable to aggregate into non-bioactive or gray elemental selenium, which limits their application in the biomedicine field. This study aimed to prepare stable SeNPs by using lentinan (LNT) as a template and evaluate its anti-colon cancer activity. The average particle diameter of obtained lentinan-selenium nanoparticles (LNT-SeNPs) was approximately 59 nm and presented zero-valent, amorphous, and spherical structures. The monodisperse SeNPs were stabilized by LNT through hydrogen bonding interactions. LNT-SeNPs solution remained highly stable at 4°C for at least 8 weeks. The stability of LNT-SeNPs solution sharply decreased under high temperature and strong acidic conditions. LNT-SeNPs showed no obvious cytotoxic effect on normal cells (IEC-6) but significantly inhibited the proliferation of five colon cancer cells (HCT-116, HT-29, Caco-2, SW620, and CT26). Among them, LNT-SeNPs exhibited the highest sensitivity toward HCT-116 cells with an IC value of 7.65 μM. Also, LNT-SeNPs displayed better cancer cell selectivity than sodium selenite and selenomethionine. Moreover, LNT-SeNPs promoted apoptosis of HCT-116 cells through activating mitochondria-mediated apoptotic pathway. Meanwhile, LNT-SeNPs induced cell cycle arrest at G0/G1 phase in HCT-116 cells modulation of cell cycle regulatory proteins. The results of this study indicated that LNT-SeNPs possessed strong potential application in the treatment of colorectal cancer (CRC).
硒纳米颗粒（SeNPs）因其优异的生物活性和低毒性而受到广泛关注。然而，SeNPs极易聚集成无生物活性的或灰色的元素硒，这限制了它们在生物医学领域的应用。本研究旨在以香菇多糖（LNT）为模板制备稳定的SeNPs，并评估其抗结肠癌活性。所制备的香菇多糖-硒纳米颗粒（LNT-SeNPs）的平均粒径约为59nm，呈现零价、无定形和球形结构。单分散的SeNPs通过氢键相互作用被LNT稳定。LNT-SeNPs溶液在4℃下至少8周保持高度稳定。在高温和强酸性条件下，LNT-SeNPs溶液的稳定性急剧下降。LNT-SeNPs对正常细胞（IEC-6）无明显细胞毒性作用，但显著抑制了五种结肠癌细胞（HCT-116、HT-29、Caco-2、SW620和CT26）的增殖。其中，LNT-SeNPs对HCT-116细胞表现出最高的敏感性，IC值为7.65μM。此外，LNT-SeNPs比亚硒酸钠和硒代蛋氨酸表现出更好的癌细胞选择性。此外，LNT-SeNPs通过激活线粒体介导的凋亡途径促进HCT-116细胞凋亡。同时，LNT-SeNPs通过调节细胞周期调节蛋白诱导HCT-116细胞的细胞周期停滞在G0/G1期。本研究结果表明，LNT-SeNPs在结直肠癌（CRC）治疗中具有强大的潜在应用价值。
