Tiss Mohamed, Hamden Khaled
Laboratory of Bioresources Integrative Biology and Exploiting, Higher Institute of Biotechnology of Monastir, University of Monastir, Monastir, Tunisia.
Evid Based Complement Alternat Med. 2022 Aug 30;2022:6816942. doi: 10.1155/2022/6816942. eCollection 2022.
In this study, we attempted for the first time to determine the phytochemical compositions and biopharmaceutical properties of methanol extract (GAME) and water extract (GAWE). High-performance liquid chromatography with diode array detection (HPLC-DAD) analysis was performed to establish the chemical profile of the investigated extracts. Chemical composition analysis was taken in the presence of various bioactive compounds such as quercetin 7-O-glucoside and apigenin 7-O-glucoside in GAME. In GAWE, various abundant compounds were found in the extract such as quercetin 7-O-glucoside, apigenin, quercetin, apigenin 7-O-glucoside, and cinnamic acid. This study showed that the administration of GAWE and GAME to type 1 diabetic rats decreased fasting blood glucose, protected pancreas -cells from death and injury, increased liver glycogen rate, and ameliorated oral glucose tolerance test. Moreover, GA reduced weight loss, and diabetes decreased basic physical activity. In addition, the administration of GA extracts in diabetic rats protected from diabetes-induced liver, kidney, testes, heart, and bone toxicities. GAWE has possible value for antidiabetic oral medication.
在本研究中，我们首次尝试确定甲醇提取物（GAME）和水提取物（GAWE）的植物化学成分及生物药学特性。采用带二极管阵列检测的高效液相色谱法（HPLC-DAD）分析来确定所研究提取物的化学特征。对GAME中存在的各种生物活性化合物（如槲皮素7-O-葡萄糖苷和芹菜素7-O-葡萄糖苷）进行了化学成分分析。在GAWE中，提取物中发现了各种丰富的化合物，如槲皮素7-O-葡萄糖苷、芹菜素、槲皮素、芹菜素7-O-葡萄糖苷和肉桂酸。本研究表明，给1型糖尿病大鼠施用GAWE和GAME可降低空腹血糖，保护胰腺β细胞免于死亡和损伤，提高肝糖原率，并改善口服葡萄糖耐量试验。此外，GA减轻体重减轻，糖尿病降低基础身体活动。此外，给糖尿病大鼠施用GA提取物可预防糖尿病引起的肝脏、肾脏、睾丸、心脏和骨骼毒性。GAWE在抗糖尿病口服药物方面具有潜在价值。
