植物化学研究与 L. 的抗氧化活性
Phytochemical Investigation and Antioxidant Activity of L.
机构信息
Laboratory of Applied Biology and Pathology, Department of Biology, Faculty of Sciences of Tetouan, Abd Al-Malek Essaadi University, Tetouan 93000, Morocco.
Laboratory of Valorization of Resources and Chemical Engineering, Department of Chemistry, Abdelmalek Essaadi University, Tangier 90000, Morocco.
出版信息
Molecules. 2021 Feb 2;26(3):759. doi: 10.3390/molecules26030759.
The Moroccan flora is rich in medicinal plants that are commonly used in folk medicine for the treatment of various diseases. The present study was designed to investigate the total phenolic and flavonoid contents, as well as the antioxidant properties of leaves extracts from L. colected from the Taza region in northeast Morocco. Additionally, the individual phenolics and volatiles of the extracts were also evaluated. The organic extracts of this plant were obtained by Soxhlet extraction using two different solvents, namely ethyl acetate and chloroform. The antioxidant capacity of leaves extracts was measured using DPPH, ABTS and FRAP assays; the phenolic profile was determined by HPLC-DAD/ESI-MS analysis, whereas the volatile composition was elucidated by GC-MS. The ethyl acetate extract analysis showed a total of 20 phenolic compounds and the determination of phenolic contents showed a significant value of 56.5 ± 0.61 µg GAE/mg of extract in comparison with the chloroform extract (18.9 ± 0.48 µg GAE/mg of extract). Also, the determination of the flavonoid contents revealed that the ethyl acetate extract contained the highest value (30.2 ± 0.55 µg CE/mg of extract) in comparison with the chloroform extract (18.0 ± 0.36 µg CE/mg of extract). Concerning the antioxidant properties, interesting values were attained for the ethyl acetate extract which exhibited higher antioxidant activity, namely IC = 12.3 ± 3.83 µg/mL and IC50 = 37.0 ± 2.45 µg/mL for the DPPH and ABTS assays, respectively, whereas a value of 531.1 ± 17.08 (mg AAE/g DW) was obtained for the FRAP assay. Concerning the volatile profile, a total of 73 compounds were positively detected and among them -hexadecanoic acid (13.5%) was the most abundant one. The results achieved confirm the important role of this plant as a source of natural antioxidants.
摩洛哥的植物区系富含药用植物，这些植物在民间医学中常用于治疗各种疾病。本研究旨在研究从摩洛哥东北部塔扎地区采集的 L. 的总酚和类黄酮含量以及抗氧化特性。此外，还评估了提取物中的个体酚类和挥发性物质。使用索氏提取法用两种不同溶剂，即乙酸乙酯和氯仿，从该植物的有机提取物中提取。使用 DPPH、ABTS 和 FRAP 测定法测定叶片提取物的抗氧化能力；通过 HPLC-DAD/ESI-MS 分析确定酚类谱，通过 GC-MS 阐明挥发性成分。乙酸乙酯提取物分析表明总共有 20 种酚类化合物，酚含量的测定表明与氯仿提取物（18.9 ± 0.48 µg GAE/mg 提取物）相比，具有显著的 56.5 ± 0.61 µg GAE/mg 提取物的值。此外，类黄酮含量的测定表明，与氯仿提取物（18.0 ± 0.36 µg CE/mg 提取物）相比，乙酸乙酯提取物含有最高值（30.2 ± 0.55 µg CE/mg 提取物）。关于抗氧化特性，乙酸乙酯提取物表现出较高的抗氧化活性，获得了有趣的数值，即在 DPPH 和 ABTS 测定中，IC = 12.3 ± 3.83 µg/mL 和 IC50 = 37.0 ± 2.45 µg/mL，而 FRAP 测定中则获得了 531.1 ± 17.08（mg AAE/g DW）的值。关于挥发性谱，共检测到 73 种化合物，其中 -十六烷酸（13.5%）是最丰富的一种。所获得的结果证实了这种植物作为天然抗氧化剂来源的重要作用。
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