Kim Da-Seul, Kim Jun Hyuk, Baek Seung-Woon, Lee Jun-Kyu, Park So-Yeon, Choi Bogyu, Kim Tae-Hyung, Min Kyunghoon, Han Dong Keun
Department of Biomedical Science, CHA University, Gyeonggi-do, Republic of Korea.
School of Integrative Engineering, Chung-Ang University, Seoul, Republic of Korea.
J Tissue Eng. 2022 Sep 2;13:20417314221122089. doi: 10.1177/20417314221122089. eCollection 2022 Jan-Dec.
Tendinopathy is a term used to describe tendon disorders that are marked by pain and a loss of function. Recent studies demonstrated that inflammation plays an important role throughout the broad spectrum of tendinopathy. Conventional treatments such as steroid injections, analgesics, and physical modalities simply give pain relief and do not alter the disease progression without the tendon regeneration effect. Tenocytes are responsible for maintaining the tendon matrix and understanding how they function is essential to studying new treatments for tendinopathy. Our previous study showed the protective effects of vitamin D (Vit D) on damaged tenocytes. Besides its well-known effects on bone metabolism, the non-classical action of Vit D is the pleiotropic effects on modulating immune function. In the present study, we developed a Vit D delivery system with hyaluronic acid (HA), which is one of the major components of the extracellular matrix that has anti-inflammation and wound-healing properties. A novel Vit D delivery system with cross-linked HA hydrogel (Gel) and Tween 80 (T80), Vit D@Gel/T80, could be a new regeneration technique for the treatment of tendinopathy. Vit D@Gel/T80 reduced TNF-α induced damage to human tenocytes in vitro. In an animal study, the Vit D@Gel/T80 injected group demonstrated tendon restoration features. As a result, this Vit D@Gel/T80 system might be a local injection material in the treatment for tendinopathy.
肌腱病是一个用于描述以疼痛和功能丧失为特征的肌腱疾病的术语。最近的研究表明，炎症在广泛的肌腱病谱中起着重要作用。传统治疗方法，如类固醇注射、镇痛药和物理治疗，只是缓解疼痛，在没有肌腱再生作用的情况下并不能改变疾病的进展。肌腱细胞负责维持肌腱基质，了解它们的功能对于研究肌腱病的新治疗方法至关重要。我们之前的研究表明维生素D（Vit D）对受损的肌腱细胞有保护作用。除了其对骨代谢的众所周知的作用外，Vit D的非经典作用是对调节免疫功能的多效性作用。在本研究中，我们开发了一种含有透明质酸（HA）的Vit D递送系统，透明质酸是细胞外基质的主要成分之一，具有抗炎和伤口愈合特性。一种新型的含有交联HA水凝胶（Gel）和吐温80（T80）的Vit D递送系统，即Vit D@Gel/T80，可能是一种治疗肌腱病的新再生技术。Vit D@Gel/T80在体外减少了肿瘤坏死因子-α（TNF-α）诱导的对人肌腱细胞的损伤。在一项动物研究中，注射Vit D@Gel/T80的组表现出肌腱修复特征。因此，这种Vit D@Gel/T80系统可能是治疗肌腱病的局部注射材料。
