Luo Yu, Wang Jiating, Wang Chenfei, Wang Dongbin, Li Chengji, Zhang Bin, Zhong Xiaoting, Chen Linkang, Li Hao, Su Hongtian, Zheng Qiuyi, Zhu Dajian, Tang Huanwen, Guo Lianxian
Dongguan Key Laboratory of Environmental Medicine, School of Public Health, Guangdong Medical University, Dongguan 523808, China.
Dongguan Key Laboratory of Environmental Medicine, School of Public Health, Guangdong Medical University, Dongguan 523808, China; Guangdong Provincial Key Laboratory of Food, Nutrition and Health, Department of Epidemiology, School of Public Health, Sun Yat-sen University, Guangzhou 510080, China.
Sci Total Environ. 2023 Jan 1;854:158583. doi: 10.1016/j.scitotenv.2022.158583. Epub 2022 Sep 7.
Arsenic can be specifically enriched by rice, and the health hazards caused by high arsenic rice are gradually attracting attention. This study aimed to explore the potential of microbial detoxification via gut microbiome in the treatment of sub-chronic arsenic poisoning. We first exposed mice to high-dose arsenic feed (30 mg/kg, rice arsenic composition) for 60 days to promote arsenic-induced microbes in situ in the gastrointestinal tract, then transplanted their fecal microbiota (FMT) into another batch of healthy recipient mice, and dynamically monitored the microbial colonization by 16S rRNA sequencing and ITS sequencing. The results showed that in situ arsenic-induced fecal microbiome can stably colonized and interact with indigenous microbes in the recipient mice in two weeks, and established a more stable network of gut microbiome. Then, the recipient mice continued to receive high-dose arsenic exposure for 52 days. After above sub-chronic arsenic exposure, compared with the non-FMT group, fecal arsenic excretion, liver and plasma arsenic accumulation were significantly lower (P < 0.05), and that in kidney, hair, and thighbone present no significant differences. Metabolomics of feces- plasma-brain axis were also disturbed, some up-regulated metabolites in feces, plasma, and cerebral cortex may play positive roles for the host. Therefore, microbial detoxification has potential in the treatment of sub-chronic arsenic poisoning. However, gut flora is an extremely complex community with different microorganisms have different arsenic metabolizing abilities, and various microbial metabolites. Coupled with the matrix effects, these factors will have various effects on the efflux and accumulation of arsenic. The definite effects (detoxification or non-detoxification) could be not assured based on the current study, and more systematic and rigorous studies are needed in the future.
砷可被水稻特异性富集，高砷水稻所导致的健康危害正逐渐受到关注。本研究旨在探讨通过肠道微生物群进行微生物解毒在治疗亚慢性砷中毒方面的潜力。我们首先将小鼠暴露于高剂量砷饲料（30毫克/千克，水稻砷成分）中60天，以促进胃肠道中砷诱导的微生物原位生长，然后将它们的粪便微生物群（FMT）移植到另一批健康的受体小鼠中，并通过16S rRNA测序和ITS测序动态监测微生物定植情况。结果表明，原位砷诱导的粪便微生物群能够在两周内稳定定植于受体小鼠体内，并与本土微生物相互作用，建立起更稳定的肠道微生物群网络。然后，受体小鼠继续接受高剂量砷暴露52天。经过上述亚慢性砷暴露后，与未进行粪便微生物群移植的组相比，粪便砷排泄量、肝脏和血浆中的砷积累量显著降低（P<0.05），而肾脏、毛发和大腿骨中的砷含量无显著差异。粪便-血浆-脑轴的代谢组学也受到干扰，粪便、血浆和大脑皮层中一些上调的代谢产物可能对宿主发挥积极作用。因此，微生物解毒在治疗亚慢性砷中毒方面具有潜力。然而，肠道菌群是一个极其复杂的群落，不同的微生物具有不同的砷代谢能力以及各种微生物代谢产物。再加上基质效应，这些因素将对砷的外排和积累产生各种影响。基于目前的研究无法确定其确切作用（解毒或非解毒），未来需要更系统、更严谨的研究。
