Karaçelik Ayça Aktaş, Türkuçar Semra Alkan, Karaköse Mustafa
Department of Food Processing, Espiye Vocational School, Giresun University, 28600, Giresun, Turkey.
Department of Nutrition and Dietetics, Faculty of Health Sciences, Alanya Alaaddin Keykubat University, 07425, Alanya, Antalya, Turkey.
Chem Biodivers. 2022 Oct;19(10):e202200552. doi: 10.1002/cbdv.202200552. Epub 2022 Sep 29.
Angelica sylvestris L. var. stenoptera Avé-Lall ex Boiss. (ASS) is an endangered endemic plant to Turkey. The objective of the study was to determine antioxidant activities, total phenolics, and phytochemical properties of methanolic extracts (MEs) and essential oil (EO) from ASS for the first time with the methods of 2,2-diphenyl-1-picrylhydrazyl (DPPH⋅) radical scavenging activities, 2,2-azinobis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS⋅ ), ferric reducing/antioxidant power (FRAP), the Folin-Ciocalteu, liquid chromatography-tandem mass spectrometry (LC-MS/MS), and gas chromatography-mass spectrometry (GC-MS), respectively. The leaf extract of ASS was found to be the richest in phenolic content (543.91±6.33 GAE, μg/mL) and showed the highest DPPH⋅ and FRAP activities (IC : 0.1140±0.0011 mg/mL, 675.62±15.01 μM TEAC). EO of ASS root showed DPPH⋅ and FRAP activities (IC : 1.3248±0.0572 mg/mL, 346.67±12.75 μM TEAC). 19 phenolics were detected in MEs of different parts of ASS by LC/MS/MS. In the chemical composition of ASS root EO by GC/MS, globulol (70.70 %) was found to be the major compound. Our results indicate that ASS can be used a source of phytochemicals and antioxidants for conservation and sustainability of endangered plants.
宽翅当归属植物（Angelica sylvestris L. var. stenoptera Avé-Lall ex Boiss.，简称ASS）是土耳其特有的濒危植物。本研究的目的是首次采用2,2-二苯基-1-苦基肼自由基清除活性（DPPH·）、2,2-联氮-双-（3-乙基苯并噻唑啉-6-磺酸）（ABTS·）、铁还原/抗氧化能力（FRAP）、福林-酚法、液相色谱-串联质谱（LC-MS/MS）和气相色谱-质谱（GC-MS）等方法，测定ASS甲醇提取物（MEs）和精油（EO）的抗氧化活性、总酚含量和植物化学特性。结果发现，ASS叶提取物的酚含量最高（543.91±6.33 GAE，μg/mL），DPPH·和FRAP活性也最高（IC50：0.1140±0.0011 mg/mL，675.62±15.01 μM TEAC）。ASS根精油具有DPPH·和FRAP活性（IC50：1.3248±0.0572 mg/mL，346.67±12.75 μM TEAC）。通过LC/MS/MS在ASS不同部位的MEs中检测到19种酚类物质。通过GC/MS分析ASS根精油的化学成分，发现球松素（70.70%）是主要成分。我们的研究结果表明，ASS可作为植物化学物质和抗氧化剂的来源，用于濒危植物的保护和可持续利用。
