Biochemistry Unit, Regional Animal Health Research Laboratory, Animal Health Research Institute, El-Kharga, El-Wadi El-Gadid, 725211 Egypt.
Vet Res Commun. 2011 Jan;35(1):35-45. doi: 10.1007/s11259-010-9450-x. Epub 2010 Nov 18.
Oxidative stress is an imbalance between radical-generating and radical-scavenging activity, resulting in oxidation products and tissue damage. This study was aimed to evaluate the status of oxidative stress indices in blood of camels naturally infested with S. scabiei. Forty-seven male camels (Camelus dromedaries) were divided according to the extent of the infested area with Sarcoptes scabiei into four groups, mild (MID, n = 12), moderate (MOD, n = 10), severely infested (SEV, n = 10) and healthy control group (n = 15). Blood was used for determination of red cell count (RBC), hemoglobin (Hb), packed cell volume (PCV), serum nitric oxide (NO(•), a free radical), ascorbate and albumin concentrations, and erythrocytic values of malondialdehyde (MDA, a marker of lipid peroxidation), protein carbonyls (PC, an indicator of protein oxidation), glutathione (GSH) superoxide dismutase (SOD) and catalase (CAT). Decreased levels (P < 0.05) of RBC, Hb, PCV, albumin and ascorbate were noticed in MOD and SEV compared to controls with the lowest values (P < 0.05) in SEV except for ascorbate, where MOD did not differ from SEV. Compared to controls, NO(•) gradually increased (P < 0.05) in MID followed by MOD and SEV, whereas MDA and PC were higher (P < 0.05) in MOD and SEV. PC was higher (P < 0.05) in MOD than SEV. In addition, the antioxidants GSH, SOD and CAT were higher (P < 0.05) in MID and lower (P < 0.05) in MOD and SEV compared to controls. GSH was lower (P < 0.05) in SEV compared to MOD. Besides, Hb was negatively correlated with NO(•) (r = -0.68, P < 0.001), MDA (r = -0.53, P < 0.001) and PC (r = -0.73, P < 0.001). In conclusion, dromedary sarcoptosis is accompanied by a state of oxidative stress process, which increased by increasing the area of infestation, and may contribute to the pathogenesis of the disease.
氧化应激是自由基生成和清除活性之间的失衡，导致氧化产物和组织损伤。本研究旨在评估天然感染 Sarcoptes scabiei 的骆驼血液中的氧化应激指标状况。将 47 只雄性骆驼（单峰驼）根据感染面积分为四组，轻度（MID，n = 12）、中度（MOD，n = 10）、严重感染（SEV，n = 10）和健康对照组（n = 15）。血液用于测定红细胞计数（RBC）、血红蛋白（Hb）、红细胞压积（PCV）、血清一氧化氮（NO•）、抗坏血酸和白蛋白浓度，以及红细胞丙二醛（MDA，脂质过氧化的标志物）、蛋白羰基（PC，蛋白氧化的指标）、谷胱甘肽（GSH）超氧化物歧化酶（SOD）和过氧化氢酶（CAT）的值。与对照组相比，MOD 和 SEV 中的 RBC、Hb、PCV、白蛋白和抗坏血酸水平降低（P < 0.05），而 SEV 中的值最低（P < 0.05），除了抗坏血酸，MOD 与 SEV 没有差异。与对照组相比，MID 后逐渐升高（P < 0.05），MOD 和 SEV 逐渐升高（P < 0.05），而 MDA 和 PC 升高（P < 0.05）MOD 和 SEV。与 SEV 相比，MOD 中的 PC 更高（P < 0.05）。此外，与对照组相比，MID 和 MOD 及 SEV 中的抗氧化剂 GSH、SOD 和 CAT 更高（P < 0.05），而 MOD 和 SEV 中的 GSH 更低（P < 0.05）。与 MOD 相比，SEV 中的 GSH 更低（P < 0.05）。此外，Hb 与 NO•（r = -0.68，P < 0.001）、MDA（r = -0.53，P < 0.001）和 PC（r = -0.73，P < 0.001）呈负相关。总之，单峰驼的 sarcoptosis 伴随着氧化应激过程的状态，随着感染面积的增加而增加，可能有助于疾病的发病机制。
