Department of Bioengineering and Technology, College of Chemical Engineering, Huaqiao University, Xiamen, Fujian, 361021, People's Republic of China.
Department of Environmental Science and Engineering, College of Chemical Engineering, Huaqiao University, Xiamen, Fujian, 361021, People's Republic of China.
Environ Sci Pollut Res Int. 2023 Jan;30(4):10955-10968. doi: 10.1007/s11356-022-22879-z. Epub 2022 Sep 10.
Biosynthetic of silver nanoparticles (AgNPs) by using fungi has attracted much attention due to its high catalytic efficiency and environmentally friendly characteristic. However, a few studies have focused on the ecological toxicity effects of biogenic AgNPs on algae. Here, we first investigated the catalytic reduction of 4-nitrophenol (4-NP) by WZ07-AgNPs biosynthesized by Letendraea sp. WZ07. WZ07-AgNPs had significant catalytic activity with 97.08% degradation of 4-NP in 3.5 min. Then, the toxic effects of WZ07-AgNPs and commercial-AgNPs were compared by Chlorella pyrenoidosa growth, chlorophyll content, protein content, physiological, and biochemical indexes. The results demonstrated that the algal cell biomass of C. pyrenoidosa was differentially inhibited after exposure to different concentrations of AgNPs, which showed concentration dependence and time dependence. The 96h-EC values of WZ07-AgNPs and commercial-AgNPs on C. pyrenoidosa were 15.99 mg/mL and 12.69 mg/mL, respectively. With the increase concentration of AgNPs, the chlorophyll content was gradually decreased, the protein content was first increased and then decreased, the activities of superoxide dismutase (SOD) and catalase (CAT) were decreased, and the level of malondialdehyde (MDA) was increased significantly of C. pyrenoidosa. In general, AgNPs affect the growth of algae to some extent. However, compared with commercial-AgNPs, WZ07-AgNPs is less toxic to C. pyrenoidosa, which could be used as a potential and an eco-friendly catalyst. This study provides a basis for the safe application of biosynthetic AgNPs.
真菌生物合成的银纳米粒子（AgNPs）因其高催化效率和环境友好特性而备受关注。然而，很少有研究关注生物合成的 AgNPs 对藻类的生态毒性效应。在这里，我们首次研究了 Letendraea sp. WZ07 生物合成的 WZ07-AgNPs 对 4-硝基苯酚（4-NP）的催化还原。WZ07-AgNPs 具有显著的催化活性，可在 3.5 分钟内将 4-NP 降解 97.08%。然后，通过蛋白核小球藻的生长、叶绿素含量、蛋白质含量、生理和生化指标比较了 WZ07-AgNPs 和商业-AgNPs 的毒性效应。结果表明，AgNPs 的不同浓度暴露后，蛋白核小球藻的藻细胞生物量受到不同程度的抑制，表现出浓度依赖性和时间依赖性。WZ07-AgNPs 和商业-AgNPs 对蛋白核小球藻的 96h-EC 值分别为 15.99 mg/mL 和 12.69 mg/mL。随着 AgNPs 浓度的增加，叶绿素含量逐渐降低，蛋白质含量先升高后降低，超氧化物歧化酶（SOD）和过氧化氢酶（CAT）的活性降低，丙二醛（MDA）水平显著升高。总的来说，AgNPs 对藻类的生长有一定的影响。然而，与商业-AgNPs 相比，WZ07-AgNPs 对蛋白核小球藻的毒性较小，可作为一种潜在的环保型催化剂。本研究为生物合成 AgNPs 的安全应用提供了依据。
